Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs
| Autor: | Karen Beckett, Jean-Paul Vincent, Lucy Palmer, Isabelle Gaugue, Ruth H. Palmer, Satoshi Kakugawa, Yasuo Yamazaki, Cyrille Alexandre |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Endosome Ubiquitin-Protein Ligases Green Fluorescent Proteins Signalling Endosomes Wnt1 Protein Biology Endocytosis Exocytosis Article Animals Genetically Modified 03 medical and health sciences Wnt 0302 clinical medicine Animals Drosophila Proteins Wings Animal In Situ Hybridization Fluorescence Epithelial polarity Microscopy Confocal integumentary system fungi Wnt signaling pathway Cell Biology 3. Good health Cell biology Imaginal disc Ubiquitin ligase 030104 developmental biology Drosophila melanogaster Transcytosis Imaginal Discs RNA Interference Epithelia 030217 neurology & neurosurgery Drosophila Protein Intracellular trafficking Signal Transduction |
| Zdroj: | Nature cell biology |
| ISSN: | 1476-4679 1465-7392 |
| Popis: | The apical and basolateral membranes of epithelia are insulated from each other, preventing the transfer of extracellular proteins from one side to the other1. Thus, a signalling protein produced apically is not expected to reach basolateral receptors. Evidence suggests that Wingless, the main Drosophila Wnt is secreted apically in the embryonic epidermis2, 3. However, in the wing imaginal disc epithelium, Wingless is mostly seen on the basolateral membrane where it spreads from secreting to receiving cells 4, 5. Here we examine the apico-basal movement of Wingless in Wingless-producing cells of wing imaginal discs. We find that it is presented first on the apical surface before making its way to the basolateral surface, where it is released and allowed to interact with signalling receptors. We show that Wingless transcytosis involves Dynamin-dependent endocytosis from the apical surface. Subsequent trafficking from early apical endosomes to the basolateral surface requires Godzilla, a member of the RNF family of membrane-anchored E3 ubiquitin ligases. Without such transport, Wingless signalling is strongly reduced in this tissue. |
| Databáze: | OpenAIRE |
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