Inhibition of colon cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA of the Ki-ras gene
| Autor: | T Sakakibara, Imanishi T, Chouhei Sakakura, Toshio Takahashi, T. Oyama, M Ogaki, K Ozaki, Hagiwara A, Junya Yamazaki, Hiroyuki Tsujimoto |
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| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Colorectal cancer Blotting Western Molecular Sequence Data Antineoplastic Agents Proto-Oncogene Mas Sense (molecular biology) Tumor Cells Cultured medicine Humans Pharmacology (medical) RNA Messenger RNA Neoplasm Pharmacology Regulation of gene expression Messenger RNA Base Sequence Oligonucleotide Cell growth Chemistry RNA Oligonucleotides Antisense medicine.disease Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic RNA silencing Genes ras Oncology Colonic Neoplasms Cell Division |
| Zdroj: | Anti-Cancer Drugs. 6:553-561 |
| ISSN: | 0959-4973 |
| DOI: | 10.1097/00001813-199508000-00008 |
| Popis: | Point mutations that activate the Ki-ras proto-oncogene are present in approximately 50% of human colorectal tumors and the activated Ki-ras gene is considered to play an important role in colorectal cancer cell proliferation. Five different colon cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of Ki-ras. Treatment with antisense oligonucleotides at concentrations between 10 and 40 microM significantly and dose-dependently inhibited cell growth, colony formation and Ki-ras protein production of the colon cancer cells with activated Ki-ras, but did not affect the normal cells and colon cancer cells without Ki-ras mutation. These results show that use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behavior of human colon cancer cells with an activated Ki-ras gene. |
| Databáze: | OpenAIRE |
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