Effect of phacoemulsification fluid flow on the corneal endothelium: experimental study in rabbit eyes
| Autor: | Phillip Qu, Randall J. Olson, Nick Mamalis, Jacob T Harris, Ben J Brintz, Catherine Culp, Emilie L Ungricht, Liliana Werner |
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| Rok vydání: | 2022 |
| Předmět: |
Corneal endothelium
medicine.medical_specialty genetic structures Endothelium Anterior Chamber medicine.medical_treatment Enucleation Balanced salt solution Cataract Extraction Cornea chemistry.chemical_compound Ophthalmology Animals Humans Medicine Cell damage Phacoemulsification business.industry Endothelium Corneal medicine.disease eye diseases Sensory Systems medicine.anatomical_structure chemistry Surgery Trypan blue Rabbits sense organs business |
| Zdroj: | Journal of Cataract and Refractive Surgery. 48:481-486 |
| ISSN: | 1873-4502 0886-3350 |
| Popis: | Purpose To quantify the damage to the corneal endothelium from the flow of balanced salt solution during phacoemulsification. Setting John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA. Design Experimental Study. Methods Twelve New Zealand white rabbits received bilateral surgery. Six eyes served as controls with no irrigation (incision only). In 18 eyes, the Intrepid Balanced tip of the Centurion (Alcon) Ozil handpiece was inserted into the anterior chamber for continuous irrigation at 50mL/minute with either 250 mL or 500 mL of balanced salt solution (BSS). After euthanasia and enucleation, the corneas were removed, stained with trypan blue/alizarin red, and photographed in a standardized manner (X400 photographs from 5 specific areas, and 1 overview photograph from each corneal button). The ImageJ program was used to evaluate cell damage and loss in the photographs obtained from each cornea. Results Analysis of the X400 photographs showed no statistical differences between control, 250 mL, and 500 mL groups in the percentage of intact, damaged, or lost cells (P= 0.896, 0.851, and 0.972 respectively). For the overview photos, the differences in areas of intact and damaged cells among the groups were statistically significant, likely due to the peripheral areas of damage related to touches between the phaco tip and the endothelium during irrigation. Conclusion The volume of BSS flow alone does not appear to be a major contributing source of endothelial cell damage and loss, providing further insight on mechanisms of corneal endothelium damage during phacoemulsification. |
| Databáze: | OpenAIRE |
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