Turnover of Acinar and Islet Cells in the Pancreas of Monosodium Glutamate-Treated Obese Mice
| Autor: | Daiju Nakayama, Takayuki Yoshimoto, Fuminori Moriyasu, Junichiro Mizuguchi, Takeshi Azuma, Hideto Inokuchi, Keiichi Kawai, Yasushi Magami, Takanori Hattori, Junko H. Ohyashiki, Masaya Furukawa |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Monosodium glutamate Endocrinology Diabetes and Metabolism medicine.medical_treatment Central nervous system Medicine (miscellaneous) Tritium Glucagon Islets of Langerhans Mice chemistry.chemical_compound Endocrinology Internal medicine Sodium Glutamate medicine Animals Insulin Obesity Pancreas Mice Inbred ICR business.industry Public Health Environmental and Occupational Health Immunohistochemistry Kinetics Somatostatin medicine.anatomical_structure Animals Newborn chemistry Toxicity business Cell Division Food Science Hormone |
| Zdroj: | Obesity Research. 11:87-94 |
| ISSN: | 1071-7323 |
| DOI: | 10.1038/oby.2003.14 |
| Popis: | Objective: Subcutaneous administrations of monosodium glutamate (MSG) to neonatal animals result in obesity and induce the toxicity on the central nervous system, and furthermore, have an effect on entero-pancreatic hormone. The effect of MSG on the cell turnover of organs, especially the pancreas, has received little attention until now. This study was designed to examine the effect of MSG on pancreatic cell turnover by immunohistochemistry and [3H]thymidine autoradiography. Research Methods and Procedures: Male JcI-ICR strain mice were SC injected with MSG (2 mg/g body weight daily) for 5 days after birth, received 112 repeated injections of [3H]thymidine at 6-hour intervals for 28 days after birth, and then were killed immediately thereafter, or 30, 60, or 120 days after the last injection. Autoradiography was performed on sections immunostained for glucagon, insulin, and somatostatin. Results: After continuous labeling, most pancreatic cells were labeled, and thereafter, labeling of cells decreased in control and MSG-treated mice. The mean grain counts of acinar cells in MSG-treated mice decreased more slowly than those in control mice. On the other hand, those of islet cells, including glucagon, insulin, and somatostatin cells, decreased more rapidly in MSG-treated mice than those in control mice. Discussion: Cell turnover of acinar cells was decelerated and that of islet cells including glucagon, insulin, and somatostatin cells was accelerated in MSG-treated mice pancreas. MSG-induced hypothalamic lesions exert the contrary influences on the cell turnover of acinar and islet cells. |
| Databáze: | OpenAIRE |
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