High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process
Autor: | Valquiria Linck Bassani, Caroline Reginatto, Bruna Campos de Souza, Eloane Malvessi, Analia Borges Folle, Mauricio Moura da Silveira, Natália Moreno Conceição Beraldo, Tomás Augusto Polidoro, Maria Gabriele Delagustin, Sabrina Carra, Daniela Cauzzi Rodrigues |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Alginates Bioconversion Bioengineering Disaccharides 01 natural sciences Zymomonas mobilis Catalysis Calcium Chloride chemistry.chemical_compound 010608 biotechnology Chromatography High Pressure Liquid Zymomonas Chromatography biology 010405 organic chemistry Temperature Fructose General Medicine Cells Immobilized Hydrogen-Ion Concentration biology.organism_classification Bioproduction Lactobionic acid Culture Media 0104 chemical sciences chemistry Biocatalysis Sorbitol Industrial and production engineering Biotechnology |
Zdroj: | Bioprocess and Biosystems Engineering. 43:1265-1276 |
ISSN: | 1615-7605 1615-7591 |
Popis: | Lactobionic acid and sorbitol are produced from lactose and fructose in reactions catalyzed by glucose-fructose oxidoreductase and glucono-δ-lactonase, periplasmic enzymes present in Zymomonas mobilis cells. Considering the previously established laboratory-scale process parameters, the bioproduction of lactobionic acid was explored to enable the transfer of this technology to the productive sector. Aspects such as pH, temperature, reuse and storage conditions of Ca-alginate immobilized Z. mobilis cells, and large-scale bioconversion were assessed. Greatest catalyst performance was observed between pH range of 6.4 and 6.8 and from 39 to 43 °C. The immobilized biocatalyst was reused for twenty three 24-h batches preserving the enzymatic activity. The activity was maintained during biocatalyst storage for up to 120 days. Statistically similar results, approximately 510 mmol/L of lactobionic acid, were attained in bioconversion of 0.2 and 3.0 L, indicating the potential of this technique of lactobionic acid production to be scaled up to the industrial level. |
Databáze: | OpenAIRE |
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