Montelukast inhibition of resting and GM-CSF-stimulated eosinophil adhesion to VCAM-1 under flow conditions appears independent of cysLT1R antagonism
| Autor: | Alexander J. Robinson, Garry Michael Walsh, Vivienne Williams, Dmitry Kashanin, Frank O'Dowd |
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| Rok vydání: | 2008 |
| Předmět: |
Cyclopropanes
Immunology Vascular Cell Adhesion Molecule-1 Acetates Sulfides Biology Proinflammatory cytokine chemistry.chemical_compound Cell Adhesion medicine Humans Immunology and Allergy VCAM-1 Cell adhesion Receptor Receptors Leukotriene Antagonist Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Cell Biology Adhesion Eosinophil Molecular biology Eosinophils medicine.anatomical_structure Biochemistry chemistry Quinolines Leukotriene Antagonists Antagonism |
| Zdroj: | Journal of Leukocyte Biology. 83:1522-1529 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.1007717 |
| Popis: | Montelukast (MLK) is a cysteinyl leukotriene receptor-1 (cysLT1R) antagonist with inhibitory effects on eosinophils, key proinflammatory cells in asthma. We assessed the effect of MLK on resting and GM-CSF-stimulated eosinophil adhesion to recombinant human (rh)VCAM-1 at different flow rates using our novel microflow system. At 1 or 2 dyn cm−2, shear-stress unstimulated eosinophils tethered immediately to rhVCAM-1, “rolled” along part of the channel until they tethered, or rolled without tethering. At flow rates greater than 2 dyn cm−2, adherent eosinophils began to be displaced from rhVCAM-1. MLK (10 nM and 100 nM) gave partial (∼40%) but significant (P |
| Databáze: | OpenAIRE |
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