Precursor-size components are the basic collagenous subunits of murine tumor basement membrane
| Autor: | E. Carwile Leroy, T. Stokes Gramling, Peter Dehm, Marlene W. Karakashian |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Pathology
medicine.medical_specialty Lathyrism Basement Membrane chemistry.chemical_compound Type IV collagen Mice Rheumatology Murine tumor medicine Molecule Animals Amino Acids Basement membrane Disulfide bond Mice Inbred C57BL Molecular Weight Collagen type I alpha 1 medicine.anatomical_structure Monomer chemistry Biophysics Chromatography Gel Electrophoresis Polyacrylamide Gel Female Collagen Densitometry |
| Zdroj: | Collagen and related research. 2(1) |
| ISSN: | 0174-173X |
| Popis: | The acid-insoluble fraction of murine tumor basement membrane produced by the EHS sarcoma was extracted following reduction of disulfide bonds under nondenaturing conditions. Extracts prepared from tumors grown in either lathyritic or normal animals contain two procollagen-like chains as well as cross-linked oligomers of these chains. Extracted monomeric chains behave almost exactly like reduced, radiolabeled EHS collagen precursor chains on SDS-PAGE and SDSagarose suggesting that they are very similar collagens. Since the collagen extracted after reduction represented a significant proportion of the total tumor collagen, it is likely that tumor basement membrane is assembled from precursor-size Type IV collagen molecules. |
| Databáze: | OpenAIRE |
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