Butyrate regulates the expression of c-Src and focal adhesion kinase and inhibits cell invasion of human colon cancer cells
| Autor: | Ying Tai Jin, Hsiu Shan Yu, Yen Jen Chen, Ming Chei Maa, Jenq Chang Lee, Shan Tair Wang, Jung Hui Wang, Yuan Liu, Tzeng Horng Leu, Chia Kuang Yen |
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| Rok vydání: | 2005 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Cancer Research Transcription Genetic Proto-Oncogene Proteins pp60(c-src) Cell Cycle Proteins Butyrate Biology Focal adhesion Histones Downregulation and upregulation Cell Movement Cell Line Tumor Humans Neoplasm Invasiveness RNA Messenger Phosphorylation Molecular Biology Cell Proliferation Matrigel Cell growth Acetylation Protein-Tyrosine Kinases Cell biology Up-Regulation Gene Expression Regulation Neoplastic Butyrates Matrix Metalloproteinase 9 Cell culture Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Colonic Neoplasms Matrix Metalloproteinase 2 Tyrosine kinase Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Molecular carcinogenesis. 43(4) |
| ISSN: | 0899-1987 |
| Popis: | Epidemiological studies indicate that dietary fiber-derived fermentation products such as butyrate can prevent colon cancer development. To further dissect the role of butyrate in anticarcinogenesis, its effect on cellular growth and invasion as well as the expression of c-Src and FAK, two mutually interactive nonreceptor tyrosine kinases, in three different human colon cancer cell lines (Caco-2, SW480, and SW620) were investigated. In addition to growth inhibition, butyrate treatment results in a significant downregulation of c-Src and FAK in human colon cancer cells, which can be attributable to their reduced transcripts and implicates the participation of a butyrate-sensitive pathway in modulating their expression. Concurrent to butyrate-reduced c-Src and FAK expression is the decrease of FAK Tyr-decrease 397 phosphorylation. Besides, butyrate also abolished the secretion of MMP-2 and MMP-9. And these butyrate-mediated effects severely impaired invasion of SW620 cells through Matrigel in vitro. Interestingly, in situ parallel enhancement of c-Src and FAK was also observed in human colorectal tumor specimens. These results imply that by virtue of suppression of c-Src and FAK along with other butyrate targets in colonocytes, butyrate could effectively inhibit tumor growth and invasion. © 2005 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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