Nitrogen- and phosphorus-starved Triticum aestivum show distinct belowground microbiome profiles

Autor:	Charles W. Greer, Antoine P. Pagé, Luke Masson, Julien Tremblay
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	0106 biological sciences 0301 basic medicine Cytophagaceae Plant Science 01 natural sciences Biochemistry Comamonadaceae RNA Ribosomal 16S Conocybe Soil Microbiology Triticum Rhizosphere Multidisciplinary Ecology biology Ascomycota Microbiota Eukaryota food and beverages Phosphorus Agriculture Genomics Plants Nucleic acids Ribosomal RNA Medical Microbiology Wheat Medicine Agrochemicals Soil microbiology Research Article Cell biology Cellular structures and organelles Nitrogen Science Bulk soil Microbial Genomics Microbiology Phosphorus metabolism 03 medical and health sciences Plant-Environment Interactions Botany Genetics Grasses Microbiome Fertilizers Non-coding RNA Plant Communities Sequence Assembly Tools Bacteria Plant Ecology Ecology and Environmental Sciences Organisms Biology and Life Sciences Computational Biology Sordariomycetes Genome Analysis biology.organism_classification 030104 developmental biology RNA Ribosomes 010606 plant biology & botany Mycobiome
Zdroj:	PLoS ONE, Vol 14, Iss 2, p e0210538 (2019) PLoS ONE
ISSN:	1932-6203
Popis:	Many plants have natural partnerships with microbes that can boost their nitrogen (N) and/or phosphorus (P) acquisition. To assess whether wheat may have undiscovered associations of these types, we tested if N/P-starved Triticum aestivum show microbiome profiles that are simultaneously different from those of N/P-amended plants and those of their own bulk soils. The bacterial and fungal communities of root, rhizosphere, and bulk soil samples from the Historical Dryland Plots (Lethbridge, Canada), which hold T. aestivum that is grown both under N/P fertilization and in conditions of extreme N/P-starvation, were taxonomically described and compared (bacterial 16S rRNA genes and fungal Internal Transcribed Spacers - ITS). As the list may include novel N- and/or P-providing wheat partners, we then identified all the operational taxonomic units (OTUs) that were proportionally enriched in one or more of the nutrient starvation- and plant-specific communities. These analyses revealed: a) distinct N-starvation root and rhizosphere bacterial communities that were proportionally enriched, among others, in OTUs belonging to families Enterobacteriaceae, Chitinophagaceae, Comamonadaceae, Caulobacteraceae, Cytophagaceae, Streptomycetaceae, b) distinct N-starvation root fungal communities that were proportionally enriched in OTUs belonging to taxa Lulworthia, Sordariomycetes, Apodus, Conocybe, Ascomycota, Crocicreas, c) a distinct P-starvation rhizosphere bacterial community that was proportionally enriched in an OTU belonging to genus Agrobacterium, and d) a distinct P-starvation root fungal community that was proportionally enriched in OTUs belonging to genera Parastagonospora and Phaeosphaeriopsis. Our study might have exposed wheat-microbe connections that can form the basis of novel complementary yield-boosting tools.
Databáze:	OpenAIRE
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