Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients
| Autor: | Bullorsky Eo, Erica Leonor Hofer, Honegger Ae, Mackinlay Da, Barañao Ri, Norma Alejandra Chasseing, Bordenave Rh, Mackinlay Ta |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Stromal cell Lung Neoplasms CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Mammary gland Inmunología Bone Marrow Cells Breast Neoplasms Dinoprostone Transforming Growth Factor beta Internal medicine medicine Humans Prostaglandin E2 Fibroblast Cells Cultured SOLUBLE RACTORS biology business.industry Cancer Hematology General Medicine Transforming growth factor beta Fibroblasts medicine.disease Molecular biology CANCER Fibronectins FIBROBLAST Medicina Básica medicine.anatomical_structure Cytokine Endocrinology Culture Media Conditioned biology.protein Carcinoma Squamous Cell Female Bone marrow business Cell Division medicine.drug Interleukin-1 |
| Popis: | We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1beta), transforming growth factor beta 1 (TGF-beta1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1beta, TGF-beta1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE(2) by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1beta were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57+/-221.61 pg/ml. Levels of TGF-beta1 in BCP were lower than NC values ( P |
| Databáze: | OpenAIRE |
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