Use of a prediction method for early pregnancy status utilizing receiver operating characteristic curve analysis of peripheral blood leukocyte interferon-stimulated genes in Japanese-Black cattle
| Autor: | Toh-Ichi Hirata, Kazuyoshi Hashizume, Hitomi Yoshino, Hideo Matsuda, Kosuke Iga, Toshina Ishiguro-Oonuma, T. Yamanouchi, Toru Takahashi, Yutaka Hashiyada, Keiichiro Kizaki, Noriyuki Toji |
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| Rok vydání: | 2020 |
| Předmět: |
Myxovirus Resistance Proteins
Pregnancy Tests Youden's J statistic Biology Insemination Sensitivity and Specificity Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Food Animals Predictive Value of Tests Pregnancy 2' 5'-Oligoadenylate Synthetase Leukocytes medicine Animals Cutoff Estrous cycle 030219 obstetrics & reproductive medicine Receiver operating characteristic 0402 animal and dairy science Area under the curve 04 agricultural and veterinary sciences General Medicine medicine.disease 040201 dairy & animal science Gene Expression Regulation Interferon Regulatory Factors Cytokines Gestation Cattle Female Animal Science and Zoology Interferons |
| Zdroj: | Animal Reproduction Science. 214:106283 |
| ISSN: | 0378-4320 |
| Popis: | A prediction method for early pregnancy status (pregnant or non-pregnant) in cattle that can be used within 3 weeks after insemination is desired. Interferon-stimulated genes (ISGs) in peripheral blood leukocytes (PBLs) have been examined as prediction molecules for determination of pregnancy status. Relative abundances of ISG15 and MX2 gene transcripts in PBLs were suitable biomarkers for the prediction of pregnancy status when there were assessments of Holstein cattle. In the present study, it was determined whether ISG biomarkers are applicable for predicting gestation in Japanese-Black (JB) cattle and evaluation of the applicability of receiver operating characteristic (ROC) analysis procedures for this purpose. There was assessment of the reliability of using average ISG values in PBLs collected during the estrous cycle (AVE) as a cutoff compared to the Youden index cutoff values. Application of AVE to assessment of pregnancy status in JB cattle indicated there was reliable predictions for pregnancy status when using ISG15 and MX2 values on day 21 after insemination, which coincided with the time of assessment in the previous study with Holstein cattle. The area under the curve values of the ROC curves confirmed the reliability of using ISGs to predict pregnancy from days 18 to 21 after insemination. Comparing AVE with Youden index values, there was confirmation of the accuracy of AVE for predicting gestation. The average mRNA transcript abundance values of ISG15 and MX2 may serve as excellent pregnancy biomarkers for cattle within 3 weeks of insemination. |
| Databáze: | OpenAIRE |
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