Protein kinase C in Saccharomyces cerevisiae: comparison with the mammalian enzyme
| Autor: | Masahiro Oka, Akira Kishimoto, Hiroshi Koide, Yasutomi Nishizuka, Takayuki Iwai, Kouichi Ando, Shin-Ichi Miyamoto, Kazuhito Ikeda, Kouji Ogita, Yasuo Fukami |
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| Rok vydání: | 1990 |
| Předmět: |
Molecular Sequence Data
Saccharomyces cerevisiae Arachidonic Acids Substrate Specificity Enzyme activator Cytosol Animals Amino Acid Sequence Phosphorylation Protein Kinase C Protein kinase C Diacylglycerol kinase chemistry.chemical_classification Chromatography Arachidonic Acid Multidisciplinary biology Brain Chromatography Ion Exchange biology.organism_classification Yeast Rats Myelin basic protein Enzyme Activation Kinetics Durapatite Enzyme chemistry Biochemistry biology.protein Calcium Hydroxyapatites Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 87:5011-5015 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.87.13.5011 |
| Popis: | Protein kinase C (PKC) was detected in the yeast Saccharomyces cerevisiae with bovine myelin basic protein as the phosphate acceptor. The enzyme was purified at least 500-fold by a four-step column chromatographic procedure (phenyl-Sepharose CL-4B, Mono Q, Heparin-5PW, and hydroxyapatite). The molecular mass was approximately 90 kDa, as estimated by gel-filtration analysis. Yeast PKC was activated by the simultaneous addition of Ca2+, diacylglycerol, and phosphatidylserine. Free arachidonic acid alone could activate the enzyme to some extent. However, yeast PKC did not respond significantly to tumor-promoting phorbol esters. GTP did not serve as phosphate donor. The yeast enzyme showed substrate specificity distinctly different from that of mammalian PKCs. H1 histone and protamine were poor substrates. With myelin basic protein as a model substrate, yeast PKC phosphorylated threonyl residues preferentially, whereas rat brain PKCs phosphorylated seryl residues preferentially. Further studies should elucidate the role of yeast PKC in cellular regulation and cell cycle control. |
| Databáze: | OpenAIRE |
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