Disease resistance results from foreign phytoalexin expression in a novel plant
| Autor: | Rüdiger Hain, Hans-Jörg Reif, Elvira Krause, Ruth Langebartels, Helmut Kindl, Barbara Vornam, Wilfried Wiese, Elmon Schmelzer, Peter H. Schreier, Ronald H. Stöcker, Klaus Stenzel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Nicotiana tabacum
Molecular Sequence Data Genetically modified crops Plant disease resistance Resveratrol Transfection chemistry.chemical_compound Phytoalexins Tobacco Botany RNA Messenger Plant Diseases Botrytis cinerea chemistry.chemical_classification Multidisciplinary Base Sequence biology Plant Extracts Terpenes Phytoalexin fungi Genetic transfer food and beverages DNA Plants Plants Genetically Modified biology.organism_classification Immunity Innate Plants Toxic chemistry Sesquiterpenes Acyltransferases Solanaceae |
| Zdroj: | Nature. 361:153-156 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/361153a0 |
| Popis: | Although phytoalexins have long been inferred to be important in the defence of plants against fungal infection, there are few reports showing that they provide resistance to infection. Several plants, including grapevine, synthesize the stilbene-type phytoalexin resveratrol when attacked by pathogens. Stilbenes with fungicidal potential are formed in several unrelated plant species, such as peanut (Arachis hypogaea), grapevine (Vitis vinifera) and pine (Pinus sylvestris). Stilbene biosynthesis only specifically requires the presence of stilbene synthase. Furthermore, the precursor molecules for the formation of hydroxy-stilbenes are malonyl-CoA and p-coumaroyl-CoA, both present in plants. To investigate the potential of stilbene biosynthetic genes in a strategy of engineering pathogen resistance, we isolated stilbene synthase genes from grapevine, where they are expressed at a high level, and transferred them into tobacco. We report here that regenerated tobacco plants containing these genes are more resistant to infection by Botrytis cinerea. This is, to our knowledge, the first report of increased disease resistance in transgenic plants based on an additional foreign phytoalexin. |
| Databáze: | OpenAIRE |
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