Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle
| Autor: | Reza Ghanbarpour, Morgan Bihannic, Frédéric Auvray, Pierre Châtre, Michèle Boury, Hubert Brugère, Laurent Cavalié, Eric Oswald, Jean-Yves Madec |
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| Přispěvatelé: | Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité Antibiorésistance et Virulence Bactériennes (AVB), Laboratoire de Lyon [ANSES], Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Microbiology Department, Shahid Bahonar University of Kerman, Unité Ecophysiologie et Détection Bactérienne, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Service Bactériologie et hygiène [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), This work was supported by Institut National de la Recherche Agronomique(INRA). Morgan Bihannic had a PhD fellowship from the Institut National dela Recherche Agronomique (INRA) and the French Agency for Food,Environmental and Occupational Health and Safety (Anses). We gratefullythank Patricia Martin for helpful discussions and proofreading., Taibi, Nadia, Madec, Jean-Yves, Unité Antibiorésistance et Virulence Bactériennes, Service de Bactériologie-Hygiène, CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Institut National de la Recherche Agronomique (INRA), French Agency for Food, Environmental and Occupational Health and Safety (Anses) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Diarrhea
[SDV]Life Sciences [q-bio] Population Molecular Sequence Data diarrhée Virulence Cattle Diseases medicine.disease_cause Polymerase Chain Reaction Microbiology Pathogenic Escherichia coli Gene cluster medicine Animals education Escherichia coli Escherichia coli Infections Phylogeny virulence bactérienne education.field_of_study Adhesins Escherichia coli septicemie General Veterinary biology Base Sequence Research Escherichia coli Proteins veau toxine bactérienne agneau biology.organism_classification Pathogenicity island veterinary(all) variation génétique 3. Good health facteur de pathogénicité Bacterial adhesin Médecine vétérinaire et santé animal [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie Pilin Fimbriae Bacterial biology.protein protéine pathogène Cattle Veterinary medicine and animal Health [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie escherichia coli bactérie pathogène expression des gènes |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, 2013, pp.76 Veterinary Research (45), . (2014) Veterinary Research BMC Veterinary Research, BioMed Central, 2013, pp.76 Veterinary Research, BioMed Central, 2014, 45 (1), pp.76. ⟨10.1186/s13567-014-0076-9⟩ |
| ISSN: | 1746-6148 0928-4249 1297-9716 |
| DOI: | 10.1186/s13567-014-0076-9⟩ |
| Popis: | International audience; F17 fimbriae are produced by pathogenic Escherichia coli involved in diarrhea and septicemia outbreaks in calves and lambs. These proteins result from the expression of four different clustered genes, namely f17A, f17D, f17C and f17G, encoding a pilin protein, a periplasmic protein, an anchor protein and an adhesin protein, respectively. Several variants of f17A and f17G genes have been reported and found genetically associated with typical virulence factors of bovine pathogenic E. coli strains. In this study, a new F17e-A variant, closely related to F17b-A, was identified from a collection of 58 E. coli isolates from diarrheic calves in Iran. While highly prevalent in Iranian F17-producing clinical isolates from calves, this variant was rare among E. coli from a French healthy adult bovine population, suggesting a possible association with virulence. The f17Ae gene was also found in the genome of the Shiga-like toxin variant Stx1d–producing bovine E. coli strain MHI813, and belonged to a gene cluster also encoding a new F17-G3 variant, which greatly differed from F17-G1 and F17-G2. This gene cluster was located on a pathogenicity island integrated in the tRNA pheV gene. The gene coding for a third new F17f-A variant corresponding to a combination of F17c-A and F17d-A was also identified on the pVir68 plasmid in the bovine pathogenic E. coli strain 6.0900. In conclusion, we identified three new F17-A and F17-G variants in cattle E. coli, which may also have significant impact on the development of new diagnostics and vaccination tools. |
| Databáze: | OpenAIRE |
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