Endothelial Cell Seeding of a 4-mm I.D. Polyurethane Vascular Graft
| Autor: | Charles Fields, Andrew D. Meyer, Gary L. Bowlin, Kristin J. Pawlowski, Cynthia Allen, Stanley E. Rittgers, Anthony D. Cassano, Michael Szycher |
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| Rok vydání: | 2002 |
| Předmět: |
0301 basic medicine
Materials science Small diameter Endothelium Polyurethanes Static Electricity Biomedical Engineering Femoral artery Biomaterials 03 medical and health sciences Dogs 0302 clinical medicine medicine.artery Jugular vein medicine Animals Thrombus medicine.disease Blood Vessel Prosthesis Endothelial stem cell surgical procedures operative 030104 developmental biology medicine.anatomical_structure Microscopy Electron Scanning Seeding Endothelium Vascular 030217 neurology & neurosurgery Vascular graft Biomedical engineering |
| Zdroj: | Journal of Biomaterials Applications. 17:45-70 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | We evaluated the extent (luminal coverage) of the endothelial cell (EC) lining/neointimal development and the thromboresistance of electrostatically EC seeded small diameter Chrono Flex-polyurethane vascular grafts. The evaluation consisted of harvesting autologous, canine jugular vein ECs, electrostatically seeding the polyurethane grafts (4-mm I.D., length = 6 cm) with the harvested ECs, implanting the grafts in a canine femoral artery model for four to six weeks, and excising the grafts for histological and scanning electron microscopy evaluations. Results of the histological evaluation (mid-graft region only) indicated that electrostatic EC seeding led to neointimal development and to minimal to no thrombus formation within the EC seeded grafts. The unseeded control grafts resulted in no neointimal development and substantial thrombus formation on the graft luminal surfaces. Scanning electron microscopy examination demonstrated a mature, confluent endothelium with a ‘‘cobblestone’’ appearance on the EC seeded graft luminal surface after six weeks. We conclude that electrostatic EC seeding enhanced the development of a neointima and reduced the incidence of thrombosis in polyurethane grafts implanted in a canine femoral artery model. |
| Databáze: | OpenAIRE |
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