Bacteria as solid phase in a concentration fluorescence immunoassay analysis of antibodies to surface antigens
| Autor: | Carl Waltenbaugh, William R. Schwan, James L. Duncan |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
Immunology Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay medicine.disease_cause Monoclonal antibody Bacterial cell structure Mice Antigen Escherichia coli medicine Animals Humans Immunology and Allergy Immunoassay Antigens Bacterial Mice Inbred BALB C biology Antibodies Monoclonal Reproducibility of Results biology.organism_classification Antibodies Bacterial Enterobacteriaceae Molecular biology Flagella Polyclonal antibodies Fimbriae Bacterial Urinary Tract Infections biology.protein Female Antibody Bacteria |
| Zdroj: | Journal of Immunological Methods. 126:247-252 |
| ISSN: | 0022-1759 |
| Popis: | A modified solid-phase fluorescence immunoassay was developed using bacterial cells as the solid phase to screen antibodies produced against surface antigens from a clinical isolate of Escherichia coli, strain 1–149. The bacterial solid phase was used to analyze both polyclonal and monoclonal antibodies. The bacterial concentration fluorescence immunoassay (BCFIA) showed up to 50-fold greater sensitivity in bacterial cell detection as compared to ELISA (enzyme-linked immunosorbent assay). Moreover, BCFIA was considerably faster than ELISA with uniform reproducibility. This paper demonstrates the utility of using bacteria and their surface antigens as solid-phase matrices for antibody characterization in a FIA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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