Cloning and immunobiochemical analyses on recombinant chymopapain allergen Cari p 2 showing pollen-fruit cross-reaction
| Autor: | Kuladip Jana, Moumita Sarkar, Rajat Kanti Sarkar, Angira Dasgupta, Moumita Bhowmik, Swati Gupta Bhattacharya, Sudipto Saha, Gaurab Sircar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Proteases Immunology Chymopapain Cross Reactions medicine.disease_cause Basophil degranulation Gastrointestinal epithelium Cross-reactivity Microbiology law.invention Mice Young Adult 03 medical and health sciences 0302 clinical medicine Allergen Cysteine Proteases law medicine Animals Humans Cloning Molecular Respiratory system Molecular Biology biology Allergens Immunoglobulin E Middle Aged Recombinant Proteins 030104 developmental biology Fruit biology.protein Recombinant DNA Pollen Female Food Hypersensitivity 030215 immunology |
| Zdroj: | Molecular Immunology. 137:42-51 |
| ISSN: | 0161-5890 |
| Popis: | Papaya is reported to trigger food and respiratory allergy. Here, we identified chymopapain Cari p 2 as an allergen that can sensitize atopic individuals through fruit consumption followed by respiratory hazards through pollen exposure. Recombinant Cari p 2 displayed IgE-reactivity with 78% of papaya allergic sera. rCari p 2 also displayed allergenic activity through basophil degranulation. rCari p 2 is correctly folded and showed irreversible denaturation in the melting curve. rCari p 2 displayed IgE-cross-reactivity with homologous cysteine proteases from kiwi and pineapple. Cari p 2 transcript was also detected in papaya pulps. rCari p 2 was resistant to pepsin digestion and retained IgE-reactivity after 60 minutes of pepsin digestion. In mouse model, rCari p 2 was found to elicit inflammatory responses in the lung and gastrointestinal epithelium. Hence, Cari p 2 is a newly characterized allergen with diagnostic and immunotherapeutic potential for managing allergic disorders in papaya sensitized individuals. |
| Databáze: | OpenAIRE |
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