A highly conserved host lipase deacylates oxidized phospholipids and ameliorates acute lung injury in mice
| Autor: | Robert S. Munford, Wei Jiang, Michael Goodwin, Yiwei Chu, Benkun Zou, Jianguo Tang, Danial Saleem, Mingfang Lu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Programmed cell death Mouse Lipopolysaccharide QH301-705.5 Inflammasomes Science Acute Lung Injury Phospholipid Mice Transgenic macrophage Lung injury General Biochemistry Genetics and Molecular Biology Acyloxyacyl hydrolase oxidized phospholipid Mice chemistry.chemical_compound Immunology and Inflammation inflammasome medicine Animals Biology (General) Lipase Cells Cultured Phospholipids Inflammation General Immunology and Microbiology biology Macrophages General Neuroscience acyloxyacyl hydrolase Inflammasome General Medicine In vitro chemistry Biochemistry biology.protein Medicine lipids (amino acids peptides and proteins) Hydrochloric Acid Carboxylic Ester Hydrolases Oxidation-Reduction Research Article medicine.drug |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.70938 |
| Popis: | Oxidized phospholipids have diverse biological activities, many of which can be pathological, yet how they are inactivated in vivo is not fully understood. Here, we present evidence that a highly conserved host lipase, acyloxyacyl hydrolase (AOAH), can play a significant role in reducing the pro-inflammatory activities of two prominent products of phospholipid oxidation, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine. AOAH removed the sn-2 and sn-1 acyl chains from both lipids and reduced their ability to induce macrophage inflammasome activation and cell death in vitro and acute lung injury in mice. In addition to transforming Gram-negative bacterial lipopolysaccharide from stimulus to inhibitor, its most studied activity, AOAH can inactivate these important danger-associated molecular pattern molecules and reduce tissue inflammation and injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|