Incorporation of the novel bacterial blight resistance gene Xa38 into the genetic background of elite rice variety Improved Samba Mahsuri

Autor: Gouri Sankar Laha, Lella V. Subba Rao, Jyothi Badri, D. Ladhalakshmi, Kuldeep Singh, A. Yugander, Raman Meenakshi Sundaram, Maganti Sheshu Madhav, M. S. Prasad
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Spatial Epidemiology
Introgression
Epidemiology
Selection Markers
lcsh:Medicine
Plant Science
01 natural sciences
Genome
Geographical Locations
Medicine and Health Sciences
lcsh:Science
Pathogen
Flowering Plants
Disease Resistance
Genetics
Multidisciplinary
Plant Anatomy
Eukaryota
food and beverages
Plants
Experimental Organism Systems
Research Article
Asia
Evolutionary Processes
Inflorescences
Xanthomonas
India
Virulence
Biology
Research and Analysis Methods
03 medical and health sciences
Xanthomonas oryzae
Plant and Algal Models
Grasses
Panicles
Molecular Biology Techniques
Molecular Biology
Gene
Plant Diseases
Evolutionary Biology
lcsh:R
Organisms
Biology and Life Sciences
Marker Genes
Oryza
biology.organism_classification
Background selection
030104 developmental biology
Genes
Bacterial
People and Places
Genetics of Disease
Backcrossing
Hybridization
Genetic
lcsh:Q
Rice
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 5, p e0198260 (2018)
ISSN: 1932-6203
Popis: Bacterial blight (BB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major global production constraint, particularly in irrigated and rain-fed lowland areas. Improved Samba Mahsuri (ISM) is an elite, high-yielding, fine-grain type, BB-resistant rice variety possessing three BB-resistant genes (Xa21, xa13 and xa5) and is highly popular in the southern parts of India. As the BB pathogen is highly dynamic and the evolution of pathogen virulence against the deployed resistance genes is common, we added a novel BB-resistant gene, Xa38, into ISM through marker-assisted backcross breeding (MABB) to increase the spectrum and durability of BB resistance. The breeding line PR 114 (Xa38) was used as the donor for Xa38, whereas ISM was used as the recurrent parent. Foreground selection was conducted using PCR-based gene-specific markers for the target genes, whereas background selection was conducted using a set of polymorphic SSR markers between the parents and backcrossing that continued until the third generation. Eighteen homozygous BC3F2 plants possessing all four BB-resistant genes in the homozygous state and with a recurrent parent genome (RPG) recovery of more than 92% were identified and advanced to the BC3F6 generation. These 18 backcross-derived lines (BDLs) exhibited very high level of resistance against multiple Xoo strains and displayed agro-morphological traits, grain qualities and yield levels similar to or better than those of the recurrent parent ISM.
Databáze: OpenAIRE
Externí odkaz: