Lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of the Epstein-Barr-associated gastric carcinoma cell line GT38 in vitro
| Autor: | Wu Ye, Xiuyun Shen, Jundong Zhou, Weichang Chen, Bingtuan Liu, Pengfei Liu, Fangjun Wang, Wenping Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research viruses proliferation Cell Biology 03 medical and health sciences 0302 clinical medicine RNA interference Immunology and Microbiology (miscellaneous) lentivirus hemic and lymphatic diseases Gene expression medicine otorhinolaryngologic diseases Gene silencing gastric carcinoma Gene knockdown virus diseases latent membrane protein 2A General Medicine Articles Cell cycle Molecular biology Cell biology stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis A431 cells |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | In this study, lentivirus-mediated RNA interference (RNAi) was applied to inhibit latent membrane protein 2A (LMP2A) gene expression, in order to explore the effects of LMP2A silencing on the growth of an Epstein-Barr virus-associated gastric carcinoma (EBVaGC) cell line in vitro. Lentivirus-mediated RNAi technology was employed to specifically knock down the LMP2A gene in the EBV-positive gastric carcinoma cell line GT38. After infection, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and colony formation assays were conducted to evaluate the expression of LMP2A and the biological behavior of the GT38 cell line in vitro. The results showed that the expression of the LMP2A gene was clearly downregulated in the infected cells, which indicated that a highly efficient and stable lentivirus vector was successfully constructed. In the GT38 cells in which the expression of LMP2A was downregulated, the proliferation and colony formation of the cells was significantly inhibited. In addition, it was found that the cell cycle of the GT38 cells was arrested in the G0/G1 phase and the apoptosis rate was increased. These results indicate that lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of the EBVaGC cell line GT38 in vitro, and suggests that LMP2A is a potential target for gene therapy in the treatment of EBVaGC. |
| Databáze: | OpenAIRE |
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