The roles of ERAS during cell lineage specification of mouse early embryonic development
| Autor: | Xiaoxin Zhang, Yanhua Zhai, Zhen-Ao Zhao, Xukun Lu, Haibin Wang, Huaixiao Ma, Yang Yu, Xiaoxiao Wang, Lei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mesoderm
MAP Kinase Signaling System Immunology Down-Regulation Embryonic Development Gene Expression Germ layer Biology Oncogene Protein p21(ras) General Biochemistry Genetics and Molecular Biology Cell Line Mice mesoderm medicine Animals primitive streak Extracellular Signal-Regulated MAP Kinases cell lineage lcsh:QH301-705.5 beta Catenin Research Articles Primitive streak General Neuroscience Research Embryogenesis Gene Expression Regulation Developmental eras embryonic stem cells Embryonic stem cell Molecular biology Cell biology Gastrulation medicine.anatomical_structure lcsh:Biology (General) Gene Knockdown Techniques Mesoderm formation Reprogramming Germ Layers |
| Zdroj: | Open Biology, Vol 5, Iss 8 (2015) Open Biology |
| ISSN: | 2046-2441 |
| DOI: | 10.1098/rsob.150092 |
| Popis: | Erasencodes a Ras-like GTPase protein that was originally identified as an embryonic stem cell-specific Ras. ERAS has been known to be required for the growth of embryonic stem cells and stimulates somatic cell reprogramming, suggesting its roles on mouse early embryonic development. We now report a dynamic expression pattern ofErasduring mouse peri-implantation development: its expression increases at the blastocyst stage, and specifically decreases in E7.5 mesoderm. In accordance with its expression pattern, the increased expression ofEraspromotes cell proliferation through controlling AKT activation and the commitment from ground to primed state through ERK activation in mouse embryonic stem cells; and the reduced expression ofErasfacilitates primitive streak and mesoderm formation through AKT inhibition during gastrulation. The expression ofErasis finely regulated to match its roles in mouse early embryonic development during whichErasexpression is negatively regulated by theβ-catenin pathway. Thus, beyond its well-known role on cell proliferation, ERAS may also play important roles in cell lineage specification during mouse early embryonic development. |
| Databáze: | OpenAIRE |
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