'Pseudoclusters' and typing by random amplification of polymorphic DNA of Aspergillus fumigatus
| Autor: | K W Loudon, J P Burnie, A P Coke |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
A fumigatus
Molecular Sequence Data DNA-Directed DNA Polymerase Pathology and Forensic Medicine law.invention Microbiology Aspergillus fumigatus chemistry.chemical_compound law Humans Taq Polymerase Typing DNA Fungal Mycological Typing Techniques skin and connective tissue diseases Polymerase chain reaction DNA Primers Genetics Base Sequence biology Gene Amplification RNA-Directed DNA Polymerase General Medicine Fungi imperfecti bacterial infections and mycoses biology.organism_classification RAPD chemistry DNA Research Article Taq DNA Polymerase |
| Zdroj: | Journal of Clinical Pathology. 48:183-184 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.48.2.183 |
| Popis: | This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus. |
| Databáze: | OpenAIRE |
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