Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology
| Autor: | Marta Sánchez-Paniagua López, M. Jesús Lobo Castañón, Gloria Frutos Cabanillas, Beatriz López-Ruiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Materials science Arachis DNA Plant Molecular Sequence Data Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques Adsorption Limit of Detection Electrochemistry Humans Glycoproteins Detection limit Chromatography Base Sequence Oligonucleotide Hybridization probe Design of experiments Reproducibility of Results Self-assembled monolayer General Medicine Electrochemical Techniques Allergens Antigens Plant Ara h2 peanut allergen Linear range Electrode Calibration Nucleic Acid Conformation Electrochemical genosensor DNA Probes Food Analysis Biotechnology 2S Albumins Plant |
| Zdroj: | RUO. Repositorio Institucional de la Universidad de Oviedo instname |
| Popis: | A new selective electrochemical genosensor has been developed for the detection of an 86-mer DNA peanut sequence encoding part of the allergen Arah2(conglutin-homologprotein).The method is base on a sandwich format,which presents two advantages:it permits shortening the capture probe and avoids labeling of the target.Screen-printed gold electrodes have been used as platformfor the immobilization of oligo nucleotides by the well-knownS–Aubond.Mixedself-assembled monolayers (SAM), including thiol-modified capture probe and mercaptohexanol,were prepared to a chieve an organized, homogeneous and not too compact SAM in which unspecific adsorption of the capture probe would be prevented... This work has been cofinancied by Project MINECO-13-CTQ- 12-31157 and Project MICINN-08-CTQ-2008-02429 |
| Databáze: | OpenAIRE |
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