Assessment of liposome disruption to quantify drug delivery in vitro
Autor: | Célia F. Cruz, Ana Preto, Jaime Freitas, Ana Loureiro, Artur Cavaco-Paulo, Alexandre M. Carmo, Andreia C. Gomes, Alexandra Moreira, Patrícia Nogueira, Eugénia Nogueira |
---|---|
Přispěvatelé: | Universidade do Minho |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Drug Cytoplasm Hoechst 34 580 media_common.quotation_subject Cell Biophysics 02 engineering and technology Biochemistry in vitro methodology 03 medical and health sciences medicine Humans media_common Liposome Science & Technology Chemistry DNA Cell Biology 021001 nanoscience & nanotechnology Fluorescence In vitro 3. Good health 030104 developmental biology medicine.anatomical_structure Caco-2 Liposomes Drug delivery Immunology Benzimidazoles Liposome disruption Caco-2 Cells 0210 nano-technology |
Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
ISSN: | 0005-2736 |
DOI: | 10.1016/j.bbamem.2015.11.008 |
Popis: | Efficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose the encapsulation of the cationic dye Hoechst 34,580 to detect an increase in blue fluorescence due to its specific binding to negatively charged DNA. For that, the dye needs to be released inside the cell and translocated to the nucleus. The present approach correlates the intensity of detected fluorescent dye with liposome disruption and consequently assesses drug delivery within the cells. Eugénia Nogueira (SFRH/BD/81269/2011), Célia F. Cruz (SFRH/BD/ 100927/2014) and Ana Loureiro (SFRH/BD/81479/2011) hold scholarships from Fundação para a Ciência e a Tecnologia (FCT). This study was funded by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement NMP4-LA-2009-228827 NANOFOL. This study was also supported by FEDER through POFC – COMPETE and by national funds from FCT through the project PEst-UID/ BIA/4050/2013 and the strategic funding of ID/BIO/04469/2013 unit.We thank the Immuno-haemotherapy Department of Hospital de São João (Porto, Portugal) for providing buffy coats from healthy volunteers. |
Databáze: | OpenAIRE |
Externí odkaz: |