X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction
| Autor: | Jan-David Nicolas, Marten Bernhardt, Karl Toischer, Marius Reichardt, Tim Salditt, Charlotte Neuhaus |
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| Rok vydání: | 2020 |
| Předmět: |
Diffraction
Materials science Holography Biophysics Radiation law.invention Mice 03 medical and health sciences Electron density distribution 0302 clinical medicine Optics X-Ray Diffraction law ddc:570 Animals Myocytes Cardiac Tomography Voxel size 030304 developmental biology 0303 health sciences Tomographic reconstruction business.industry X-Rays X-ray Articles 3. Good health Phase retrieval business 030217 neurology & neurosurgery |
| Zdroj: | Biophysical journal 119(7), 1309-1323 (2020). doi:10.1016/j.bpj.2020.08.019 Biophysical Journal |
| ISSN: | 0006-3495 |
| Popis: | We present a multiscale imaging approach to characterize the structure of isolated adult murine cardiomyocytes based on a combination of full-field three-dimensional coherent x-ray imaging and scanning x-ray diffraction. Using these modalities, we probe the structure from the molecular to the cellular scale. Holographic projection images on freeze-dried cells have been recorded using highly coherent and divergent x-ray waveguide radiation. Phase retrieval and tomographic reconstruction then yield the three-dimensional electron density distribution with a voxel size below 50 nm. In the reconstruction volume, myofibrils, sarcomeric organization, and mitochondria can be visualized and quantified within a single cell without sectioning. Next, we use microfocusing optics by compound refractive lenses to probe the diffraction signal of the actomyosin lattice. Comparison between recordings of chemically fixed and untreated, living cells indicate that the characteristic lattice distances shrink by ∼10% upon fixation. |
| Databáze: | OpenAIRE |
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