Evaluation of anti-AIDS drugs in conventional mice implanted with a permeable membrane device containing human T cells infected with HIV
| Autor: | Claudy J.-P. Mullon, Karen E. Dunleavy, Lois B. Allen, Debra C. Quenelle, Kathy A. Keith, Anne D. Brazier, Beth A. Taylor, Bonnie J. Bowdon, Reginald E. Harris |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
CD4-Positive T-Lymphocytes
Anti-HIV Agents Cell Survival Polymers Ratón viruses Acrylic Resins Drug Evaluation Preclinical HIV Core Protein p24 Biology Permeability Virus Mice Peritoneal cavity Zidovudine Multiplicity of infection Cytopathogenic Effect Viral In vivo Virology medicine Animals Humans Viability assay Polyvinyl Chloride Pharmacology virus diseases Membranes Artificial Prostheses and Implants T lymphocyte HIV Reverse Transcriptase medicine.anatomical_structure HIV-1 medicine.drug |
| Zdroj: | Antiviral Research. 35:123-129 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/s0166-3542(97)00014-4 |
| Popis: | We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4+, CEM-SS cells infected with HIV 1 , strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of ∼ 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect