Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics

Autor: Li-Li Sun, Guanhua Wei, Kaixue Zhang, Cong Longfei, Zhigang Yang, Liu Zhang, Min Jiang, Gang Bai, Hong Du, Li-Yao Wang, Shu Han, Hai-Ying Bai, Menglin Yan
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Angelica sinensis
Phytochemicals
Pharmaceutical Science
chemistry.chemical_element
Calcium
Tandem mass spectrometry
Mass spectrometry
01 natural sciences
Article
Analytical Chemistry
Cell Line
Ferulic acid
lcsh:QD241-441
chemistry.chemical_compound
Metabolomics
lcsh:Organic chemistry
Tandem Mass Spectrometry
calcium antagonists
Drug Discovery
Humans
Radix
content
Physical and Theoretical Chemistry
Chromatography
High Pressure Liquid
Chromatography
biology
Geography
Molecular Structure
010405 organic chemistry
Organic Chemistry
biology.organism_classification
chemical markers
metabolomics
anti-inflammation
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Matrix-assisted laser desorption/ionization
chemistry
Chemistry (miscellaneous)
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Molecular Medicine
angelica sinensis
geoherb region
uhplc-qtof-ms/ms
Zdroj: Molecules, Vol 24, Iss 19, p 3536 (2019)
Molecules
Volume 24
Issue 19
ISSN: 1420-3049
Popis: This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.
Databáze: OpenAIRE
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