Heterogeneity in progenitor cell subtypes in the ventricular zone of the zebrafish adult telencephalon

Autor: Colette Vaillant, Martin März, Birgit Hesl, Nicolas Diotel, Chen Sok Lam, Laure Bally-Cuif, Uwe Strähle, Olivier Kah, Masanari Takamiya, Prisca Chapouton
Přispěvatelé: Institute for Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Department of Zebrafish Neurogenetics, Institute of Developmental Genetics, German Research Center for Environmental Health-Helmholtz-Zentrum München (HZM), Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Helmholtz Zentrum München = German Research Center for Environmental Health, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Telencephalon
Neurogenesis
Cellular differentiation
radial glia
Nerve Tissue Proteins
Neural Cell Adhesion Molecule L1
Biology
Animals
Genetically Modified

Nestin
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Intermediate Filament Proteins
Neuroblast
Lateral Ventricles
Animals
Progenitor cell
Zebrafish
SOX Transcription Factors
Cell Proliferation
030304 developmental biology
neural stem cells
Neurons
0303 health sciences
Neuronal Plasticity
Stem Cells
fungi
Cell Differentiation
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology

Zebrafish Proteins
biology.organism_classification
Molecular biology
Neural stem cell
adult neurogenesis
Neurology
nervous system
embryonic structures
Sialic Acids
Stem cell
Neuroglia
Biomarkers
Cell Division
030217 neurology & neurosurgery
Zdroj: Glia
Glia, Wiley, 2010, 58 (7), pp.870-88. ⟨10.1002/glia.20971⟩
Glia, 2010, 58 (7), pp.870-88. ⟨10.1002/glia.20971⟩
ISSN: 0894-1491
1098-1136
DOI: 10.1002/glia.20971⟩
Popis: International audience; The zebrafish has become a new model for adult neurogenesis, owing to its abundant neurogenic areas in most brain subdivisions. Radial glia-like cells, actively proliferating cells, and label-retaining progenitors have been described in these areas. In the telencephalon, this complexity is enhanced by an organization of the ventricular zone (VZ) in fast and slow-dividing domains, suggesting the existence of heterogeneous progenitor types. In this work, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of the adult zebrafish telencephalon (identified by expression of proliferating cell nuclear antigen (PCNA), MCM5, or bromodeoxyuridine incorporation). We demonstrate the existence of distinct populations of dividing cells at the adult telencephalic VZ. Progenitors of the overall slow-cycling domains express high levels of Sox2 and nestin:gfp as well as all glial markers tested. In contrast, domains with an overall fast division rate are characterized by low or missing expression of glial markers. PCNA-positive cells in fast domains further display a morphology distinct from radial glia and co-express PSA-NCAM, suggesting that they are early neuronal precursors. In addition, the VZ contains cycling progenitors that express neither glial markers nor nestin:gfp, but are positive for Sox2 and PSA-NCAM, identifying them as committed neuroblasts. On the basis of the marker gene expression and distinct cell morphologies, we propose a classification for the dividing cell states at the zebrafish adult telencephalic VZ.
Databáze: OpenAIRE