Heterogeneity in progenitor cell subtypes in the ventricular zone of the zebrafish adult telencephalon
Autor: | Colette Vaillant, Martin März, Birgit Hesl, Nicolas Diotel, Chen Sok Lam, Laure Bally-Cuif, Uwe Strähle, Olivier Kah, Masanari Takamiya, Prisca Chapouton |
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Přispěvatelé: | Institute for Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Department of Zebrafish Neurogenetics, Institute of Developmental Genetics, German Research Center for Environmental Health-Helmholtz-Zentrum München (HZM), Interactions cellulaires et moléculaires (ICM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Helmholtz Zentrum München = German Research Center for Environmental Health, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2010 |
Předmět: |
Telencephalon
Neurogenesis Cellular differentiation radial glia Nerve Tissue Proteins Neural Cell Adhesion Molecule L1 Biology Animals Genetically Modified Nestin 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Intermediate Filament Proteins Neuroblast Lateral Ventricles Animals Progenitor cell Zebrafish SOX Transcription Factors Cell Proliferation 030304 developmental biology neural stem cells Neurons 0303 health sciences Neuronal Plasticity Stem Cells fungi Cell Differentiation [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Zebrafish Proteins biology.organism_classification Molecular biology Neural stem cell adult neurogenesis Neurology nervous system embryonic structures Sialic Acids Stem cell Neuroglia Biomarkers Cell Division 030217 neurology & neurosurgery |
Zdroj: | Glia Glia, Wiley, 2010, 58 (7), pp.870-88. ⟨10.1002/glia.20971⟩ Glia, 2010, 58 (7), pp.870-88. ⟨10.1002/glia.20971⟩ |
ISSN: | 0894-1491 1098-1136 |
DOI: | 10.1002/glia.20971⟩ |
Popis: | International audience; The zebrafish has become a new model for adult neurogenesis, owing to its abundant neurogenic areas in most brain subdivisions. Radial glia-like cells, actively proliferating cells, and label-retaining progenitors have been described in these areas. In the telencephalon, this complexity is enhanced by an organization of the ventricular zone (VZ) in fast and slow-dividing domains, suggesting the existence of heterogeneous progenitor types. In this work, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of the adult zebrafish telencephalon (identified by expression of proliferating cell nuclear antigen (PCNA), MCM5, or bromodeoxyuridine incorporation). We demonstrate the existence of distinct populations of dividing cells at the adult telencephalic VZ. Progenitors of the overall slow-cycling domains express high levels of Sox2 and nestin:gfp as well as all glial markers tested. In contrast, domains with an overall fast division rate are characterized by low or missing expression of glial markers. PCNA-positive cells in fast domains further display a morphology distinct from radial glia and co-express PSA-NCAM, suggesting that they are early neuronal precursors. In addition, the VZ contains cycling progenitors that express neither glial markers nor nestin:gfp, but are positive for Sox2 and PSA-NCAM, identifying them as committed neuroblasts. On the basis of the marker gene expression and distinct cell morphologies, we propose a classification for the dividing cell states at the zebrafish adult telencephalic VZ. |
Databáze: | OpenAIRE |
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