Alleviation of Oxidative Damage in Multiple Tissues in Rats with Streptozotocin-Induced Diabetes by Rice Bran Oil Supplementation
| Autor: | Shyh Hsiang Lin, Huei Ju Cheng, Rong Hong Hsieh, Chia Wen Chen, Hsing Hsien Cheng, Li Ming Lien |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Oxidative phosphorylation Biology medicine.disease_cause Rice Bran Oil Streptozocin General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Rats Sprague-Dawley chemistry.chemical_compound History and Philosophy of Science Internal medicine Diabetes mellitus medicine Animals Plant Oils Deoxyguanosine chemistry.chemical_classification Reactive oxygen species General Neuroscience Rice bran oil 8-Hydroxy-2'-deoxyguanosine DNA Streptozotocin medicine.disease Rats Oxidative Stress Endocrinology chemistry Biochemistry 8-Hydroxy-2'-Deoxyguanosine Dietary Supplements Oxidative stress medicine.drug |
| Zdroj: | Annals of the New York Academy of Sciences. 1042:365-371 |
| ISSN: | 0077-8923 |
| DOI: | 10.1196/annals.1338.061 |
| Popis: | The possibility of 8-hydroxy-2'-deoxyguanosine (8-OHdG) serving as a sensitive biomarker of oxidative DNA damage and oxidative stress was investigated. Reactive oxygen species (ROS) have been reported to be a cause of diabetes induced by chemicals such as streptozotocin (STZ) in experimental animals. In this study, we examined oxidative DNA damage in multiple tissues in rats with STZ-induced diabetes by measuring the levels of 8-OHdG in the liver, kidney, pancreas, brain, and heart. Levels of 8-OHdG in mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) were also determined in multiple tissues of rats treated with rice bran oil. Levels were 0.19 +/- 0.07, 0.88 +/- 0.30, 1.97 +/- 0.05, and 9.79 +/- 3.09 (1/10(5) dG) in the liver of nDNA of normal rats, nDNA of STZ-induced diabetic rats, mtDNA of normal rats, and mtDNA of STZ-induced diabetic rats, respectively. Levels of mtDNA of 8-OHdG were 10 times higher than those of nDNA in multiple tissues. Significant reductions in mtDNA 8-OHdG levels were seen in the liver, kidney, and pancreas of diabetic rats treated with rice bran oil compared with diabetic rats without intervention. Our study demonstrated that oxidative mtDNA damage may occur in multiple tissues of STZ-induced diabetics rats. Intervention with rice bran oil treatment may reverse the increase in the frequency of 8-OHdG. |
| Databáze: | OpenAIRE |
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