SOX2 regulates acinar cell development in the salivary gland

Autor: Noel Cruz-Pacheco, Sarah M. Knox, Carlos Lizama, Yin Shen, Ann C. Zovein, Lenka Maliskova, Alison J May, Elaine Emmerson, Sara Nathan, Marcus O. Muench
Rok vydání: 2017
Předmět:
0301 basic medicine
acinar cells
Mouse
Organogenesis
Cellular differentiation
SOX2
Acinar Cells
parasympathetic nerves
Salivary Glands
Gene Knockout Techniques
Mice
Biology (General)
Salivary gland
General Neuroscience
epithelial morphogenesis
Cell Differentiation
General Medicine
3. Good health
Cell biology
medicine.anatomical_structure
embryonic structures
Adenomere
Medicine
Stem cell
Research Article
medicine.medical_specialty
QH301-705.5
Ductal cells
organogenesis
Science
Biology
digestive system
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
stomatognathic system
Internal medicine
Journal Article
medicine
Acinar cell
Animals
Humans
Progenitor cell
General Immunology and Microbiology
SOXB1 Transcription Factors
Developmental Biology and Stem Cells
030104 developmental biology
Endocrinology
Zdroj: eLife, Vol 6 (2017)
Emmerson, E, May, A J, Nathan, S, Cruz-Pacheco, N, Lizama, C O, Maliskova, L, Zovein, A C, Shen, Y, Muench, M O & Knox, S M 2017, ' SOX2 regulates acinar cell development in the salivary gland ', eLIFE, vol. 6, e26620 . https://doi.org/10.7554/eLife.26620
eLife
Emmerson, E; May, AJ; Nathan, S; Cruz-Pacheco, N; Lizama, CO; Maliskova, L; et al.(2017). SOX2 regulates acinar cell development in the salivary gland. ELIFE, 6. doi: 10.7554/eLife.26620. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/053621ww
ISSN: 2050-084X
DOI: 10.7554/elife.26620
Popis: Acinar cells play an essential role in the secretory function of exocrine organs. Despite this requirement, how acinar cells are generated during organogenesis is unclear. Using the acini-ductal network of the developing human and murine salivary gland, we demonstrate an unexpected role for SOX2 and parasympathetic nerves in generating the acinar lineage that has broad implications for epithelial morphogenesis. Despite SOX2 being expressed by progenitors that give rise to both acinar and duct cells, genetic ablation of SOX2 results in a failure to establish acini but not ducts. Furthermore, we show that SOX2 targets acinar-specific genes and is essential for the survival of acinar but not ductal cells. Finally, we illustrate an unexpected and novel role for peripheral nerves in the creation of acini throughout development via regulation of SOX2. Thus, SOX2 is a master regulator of the acinar cell lineage essential to the establishment of a functional organ. DOI: http://dx.doi.org/10.7554/eLife.26620.001
eLife digest The salivary glands produce fluid that contains enzymes to help us to digest our food. These glands contain a tree-like network of cells – known as acinar cells – that produce the fluid, and cells that form ducts to transport the fluid out of the glands. Both types of cells form from stem cells as animal embryos develop. Like all developing organs, the salivary glands receive many different signals that guide how they grow. However, the identity of the cues that instruct a stem cell to produce a new acinar cell or duct cell are not known. Emmerson et al. studied how the salivary glands develop in mouse embryos. The experiments show that a protein called SOX2 – which is an essential regulator of stem cells in embryos – is required for acinar cells to form. Loss of SOX2 inhibited the production of acinar but not duct cells. Furthermore, nerves that surround the gland provide support to cells that produce SOX2 and promote the formation of acinar cells. Further experiments suggest that the nerves also play the same role in humans. Adult organs often use developmental signals to repair or regenerate tissue. As such, understanding how an organ develops may lead to new therapies that can stimulate salivary glands and other organs to regenerate after they have been damaged in adults. DOI: http://dx.doi.org/10.7554/eLife.26620.002
Databáze: OpenAIRE
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