Phytochemical and Biological Activities of Pseudocalymma elegans: A False Garlic
| Autor: | Muhammad Ali Versiani, Khurshid Jawaid, Shaheen Faizi, Huma Khan, Sana Wahid, Maryam Shafique, Sajid Jahangir |
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| Rok vydání: | 2017 |
| Předmět: |
Antifungal Agents
Phytochemicals Ethyl acetate Molecular Conformation Bioengineering Microbial Sensitivity Tests Gram-Positive Bacteria 01 natural sciences Biochemistry Antioxidants chemistry.chemical_compound Magnoliopsida Structure-Activity Relationship Ursolic acid Gram-Negative Bacteria Organic chemistry Butylated hydroxytoluene Molecular Biology chemistry.chemical_classification Dose-Response Relationship Drug 010405 organic chemistry Diallyl disulfide Plant Extracts Fungi Fatty acid General Chemistry General Medicine Ascorbic acid 0104 chemical sciences Anti-Bacterial Agents Plant Leaves 010404 medicinal & biomolecular chemistry Diallyl trisulfide chemistry Phytochemical Molecular Medicine |
| Zdroj: | Chemistrybiodiversity. 14(10) |
| ISSN: | 1612-1880 |
| Popis: | Evaluation of phytochemical constituents and antioxidant and antimicrobial activities of hexane (PELH), dichloromethane (PELDCM), ethyl acetate (PELEA), and MeOH (PELM) extracts of young leaves of Pseudocalymma elegans have been carried out. Moreover, extracts have also been explored for the presence of sulphur containing compounds, 1,2-dithiolane (33), diallyl disulfide (35), 3-vinyl-1,2-dithiacyclohex-5-ene (37), and diallyl trisulfide (38) responsible for the garlic like smell of P. elegans. All the extracts were found to be antioxidant and showed potent inhibition with IC50 values of 0.168 ± 0.001, 0.128 ± 0.002, 0.221 ± 0.011, and 0.054 ± 0.001, respectively, as compared to standard drugs ascorbic acid (AA) and butylated hydroxytoluene (BHT). The ethyl acetate extract (PELE) showed excellent activities against few Gram-positive and Gram-negative bacteria and some fungi as compared with standard drug ceftriaxone (3rd generation cephalosporin) and nystatin, respectively. Chemical constituents of hexane, dichloromethane, and ethyl acetate extracts were identified by gas chromatography-mass spectrometry and mass spectral library search. Over all 55 chemical constituents were first time identified from the leaves which included branched and n-hydrocarbons, fatty acids, fatty acid methyl esters, fatty alcohols, terpenes, alkaloid, vitamins, glycosides, aromatic compounds, and sulfur containing compounds. Two known chemical constituents, ursolic acid (1) and β-amyrin (2), were also purified for the first time from the MeOH extract. To elucidate the structures of these compounds, UV, IR, EI-MS, 1 H- and 13 C-NMR spectroscopy were used. |
| Databáze: | OpenAIRE |
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