Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature
| Autor: | Bhaskar Jayaraman, Siji Jacob Thenumgal, Arvind Ramanathan, Arunmozhi Palaniyandi, Keerthivasan Jayakumar, Gopalakrishnan Mohan Valiathan |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Phenytoin Cancer Research Pathology medicine.medical_specialty Adolescent Epidemiology Biology medicine.disease_cause Polymerase Chain Reaction law.invention Proto-Oncogene Proteins p21(ras) Young Adult chemistry.chemical_compound Exon law medicine Humans Gene Polymerase chain reaction DNA Primers Mutation Gingival Overgrowth Gingival Carcinoma Public Health Environmental and Occupational Health DNA Neoplasm Exons Amplicon Prognosis stomatognathic diseases Cross-Sectional Studies Oncology chemistry Cancer research Anticonvulsants Female Tumor Suppressor Protein p53 DNA medicine.drug |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 13:5535-5538 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2012.13.11.5535 |
| Popis: | Background: There have been case reports of oral squamous cell carcinoma arising from gingival overgrowth induced by phenytoin - an antiepileptic drug. However, a detailed analysis for the presence of mutations in p53 and ras genes, which are the two most frequently mutated genes in cancers, in phenytoin induced gingival overgrowth tissues has hitherto not been performed. Methods: Cellular DNA isolated from twenty gingival overgrowth tissues collected from patients undergoing phenytoin therapy were amplified using primers for p53 (exons 5-8) and H-ras (exons 1-2) genes. The PCR amplicons were then gel purified and subjected to direct sequencing analysis to screen for mutations. Results: Direct sequencing of twenty samples of phenytoin induced gingival growth did not identify mutations in any of the exons of p53 and H-ras genes that were analyzed. Conclusion: Our result indicates that mutational alteration of p53 and H-ras genes is infrequent in phenytoin induced gingival growth, which thus suggests a non malignant nature of this pathology. The findings in the present study are clinically significant as a large number of epileptic patients are treated with phenytoin. |
| Databáze: | OpenAIRE |
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