Epidemiological and Virological Characteristics of Influenza Viruses Circulating in Cambodia from 2009 to 2011

Autor: Nima Asgari, Ian G. Barr, Seng Heng, Philippe Buchy, Maria Concepcion Roces, Paul F. Horwood, Naomi Komadina, Borann Sar, Ans Timmermans, Sam An Ung, Anne Kelso, Paul Kitsutani, Sowath Ly, Sok Touch, Chanthap Lon, Sek Mardy, Vannra Ieng, David Saunders, Sirenda Vong, Buth Sokhal, Sareth Rith, Srey Viseth Horm, Nora Chea, Arnaud Tarantola, Sovann Ly, Aeron C. Hurt
Přispěvatelé: Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), World Health Organization [Phnom Penh] (WHO), Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Communicable Disease Department [Phnom Penh] (CDC MOH), Ministry of Health [Mozambique], Centers for Disease Control and Prevention [Phnom Penh], Embassy of the United States of America, National Institute of Public Health [Phnom Penh], WHO Collaborating Centre for Reference and Research on Influenza, Armed Forces Research Institute of Medical Sciences [Bangkok] (AFRIMS), The study was funded by the World Health Organization office in Cambodia, the French Agency for Development (SISEA project) and by the Office of the Assistant Secretary for Preparedness and Response within the U.S. Department of Health and Human Services
Rok vydání: 2014
Předmět:
Viral Diseases
Reassortment
Viral Upper Respiratory Tract Infection
Hemagglutinin Glycoproteins
Influenza Virus
Drug resistance
medicine.disease_cause
Madin Darby Canine Kidney Cells
Influenza A Virus
H1N1 Subtype
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Medicine and Health Sciences
Influenza A virus
Antigens
Viral
Phylogeny
ComputingMilieux_MISCELLANEOUS
Multidisciplinary
biology
H1N1
Microbial Genetics
virus diseases
Orthomyxoviridae
Infectious Diseases
Influenza Vaccines
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Medicine
Seasons
Cambodia
Research Article
Science
Hemagglutinin (influenza)
Microbiology
Molecular Genetics
Dogs
Antibiotic resistance
Drug Resistance
Viral
Influenza
Human
Genetics
medicine
Animals
Humans
Point Mutation
Molecular Biology
Influenza treatment
Influenza A Virus
H3N2 Subtype
Biology and Life Sciences
biology.organism_classification
Virology
Influenza
Influenza B virus
Viral Pneumonia
Mutation
biology.protein
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Neuraminidase
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2014, 9 (10), pp.e110713. ⟨10.1371/journal.pone.0110713⟩
PLoS ONE, Vol 9, Iss 10, p e110713 (2014)
ISSN: 1932-6203
Popis: BackgroundThe Cambodian National Influenza Center (NIC) monitored and characterized circulating influenza strains from 2009 to 2011.Methodology/principal findingsSentinel and study sites collected nasopharyngeal specimens for diagnostic detection, virus isolation, antigenic characterization, sequencing and antiviral susceptibility analysis from patients who fulfilled case definitions for influenza-like illness, acute lower respiratory infections and event-based surveillance. Each year in Cambodia, influenza viruses were detected mainly from June to November, during the rainy season. Antigenic analysis show that A/H1N1pdm09 isolates belonged to the A/California/7/2009-like group. Circulating A/H3N2 strains were A/Brisbane/10/2007-like in 2009 before drifting to A/Perth/16/2009-like in 2010 and 2011. The Cambodian influenza B isolates from 2009 to 2011 all belonged to the B/Victoria lineage represented by the vaccine strains B/Brisbane/60/2008 and B/Malaysia/2506/2004. Sequences of the M2 gene obtained from representative 2009-2011 A/H3N2 and A/H1N1pdm09 strains all contained the S31N mutation associated with adamantanes resistance except for one A/H1N1pdm09 strain isolated in 2011 that lacked this mutation. No reduction in the susceptibility to neuraminidase inhibitors was observed among the influenza viruses circulating from 2009 to 2011. Phylogenetic analysis revealed that A/H3N2 strains clustered each year to a distinct group while most A/H1N1pdm09 isolates belonged to the S203T clade.Conclusions/significanceIn Cambodia, from 2009 to 2011, influenza activity occurred throughout the year with peak seasonality during the rainy season from June to November. Seasonal influenza epidemics were due to multiple genetically distinct viruses, even though all of the isolates were antigenically similar to the reference vaccine strains. The drug susceptibility profile of Cambodian influenza strains revealed that neuraminidase inhibitors would be the drug of choice for influenza treatment and chemoprophylaxis in Cambodia, as adamantanes are no longer expected to be effective.
Databáze: OpenAIRE
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