Epidemiological and Virological Characteristics of Influenza Viruses Circulating in Cambodia from 2009 to 2011
Autor: | Nima Asgari, Ian G. Barr, Seng Heng, Philippe Buchy, Maria Concepcion Roces, Paul F. Horwood, Naomi Komadina, Borann Sar, Ans Timmermans, Sam An Ung, Anne Kelso, Paul Kitsutani, Sowath Ly, Sok Touch, Chanthap Lon, Sek Mardy, Vannra Ieng, David Saunders, Sirenda Vong, Buth Sokhal, Sareth Rith, Srey Viseth Horm, Nora Chea, Arnaud Tarantola, Sovann Ly, Aeron C. Hurt |
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Přispěvatelé: | Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), World Health Organization [Phnom Penh] (WHO), Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Communicable Disease Department [Phnom Penh] (CDC MOH), Ministry of Health [Mozambique], Centers for Disease Control and Prevention [Phnom Penh], Embassy of the United States of America, National Institute of Public Health [Phnom Penh], WHO Collaborating Centre for Reference and Research on Influenza, Armed Forces Research Institute of Medical Sciences [Bangkok] (AFRIMS), The study was funded by the World Health Organization office in Cambodia, the French Agency for Development (SISEA project) and by the Office of the Assistant Secretary for Preparedness and Response within the U.S. Department of Health and Human Services |
Rok vydání: | 2014 |
Předmět: |
Viral Diseases
Reassortment Viral Upper Respiratory Tract Infection Hemagglutinin Glycoproteins Influenza Virus Drug resistance medicine.disease_cause Madin Darby Canine Kidney Cells Influenza A Virus H1N1 Subtype [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Medicine and Health Sciences Influenza A virus Antigens Viral Phylogeny ComputingMilieux_MISCELLANEOUS Multidisciplinary biology H1N1 Microbial Genetics virus diseases Orthomyxoviridae Infectious Diseases Influenza Vaccines [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine Seasons Cambodia Research Article Science Hemagglutinin (influenza) Microbiology Molecular Genetics Dogs Antibiotic resistance Drug Resistance Viral Influenza Human Genetics medicine Animals Humans Point Mutation Molecular Biology Influenza treatment Influenza A Virus H3N2 Subtype Biology and Life Sciences biology.organism_classification Virology Influenza Influenza B virus Viral Pneumonia Mutation biology.protein [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Neuraminidase |
Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (10), pp.e110713. ⟨10.1371/journal.pone.0110713⟩ PLoS ONE, Vol 9, Iss 10, p e110713 (2014) |
ISSN: | 1932-6203 |
Popis: | BackgroundThe Cambodian National Influenza Center (NIC) monitored and characterized circulating influenza strains from 2009 to 2011.Methodology/principal findingsSentinel and study sites collected nasopharyngeal specimens for diagnostic detection, virus isolation, antigenic characterization, sequencing and antiviral susceptibility analysis from patients who fulfilled case definitions for influenza-like illness, acute lower respiratory infections and event-based surveillance. Each year in Cambodia, influenza viruses were detected mainly from June to November, during the rainy season. Antigenic analysis show that A/H1N1pdm09 isolates belonged to the A/California/7/2009-like group. Circulating A/H3N2 strains were A/Brisbane/10/2007-like in 2009 before drifting to A/Perth/16/2009-like in 2010 and 2011. The Cambodian influenza B isolates from 2009 to 2011 all belonged to the B/Victoria lineage represented by the vaccine strains B/Brisbane/60/2008 and B/Malaysia/2506/2004. Sequences of the M2 gene obtained from representative 2009-2011 A/H3N2 and A/H1N1pdm09 strains all contained the S31N mutation associated with adamantanes resistance except for one A/H1N1pdm09 strain isolated in 2011 that lacked this mutation. No reduction in the susceptibility to neuraminidase inhibitors was observed among the influenza viruses circulating from 2009 to 2011. Phylogenetic analysis revealed that A/H3N2 strains clustered each year to a distinct group while most A/H1N1pdm09 isolates belonged to the S203T clade.Conclusions/significanceIn Cambodia, from 2009 to 2011, influenza activity occurred throughout the year with peak seasonality during the rainy season from June to November. Seasonal influenza epidemics were due to multiple genetically distinct viruses, even though all of the isolates were antigenically similar to the reference vaccine strains. The drug susceptibility profile of Cambodian influenza strains revealed that neuraminidase inhibitors would be the drug of choice for influenza treatment and chemoprophylaxis in Cambodia, as adamantanes are no longer expected to be effective. |
Databáze: | OpenAIRE |
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