Unexpected synergistic and antagonistic antibiotic activity against Staphylococcus biofilms
| Autor: | S P MacKenzie, Peter J. Gwynne, A. H. R. W. Simpson, Maurice P. Gallagher, G. F. Dall, S.T.J. Tsang, Steffen J. Breusch |
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| Rok vydání: | 2018 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Microbiology (medical) medicine.drug_class Joint Prosthesis Staphylococcus 030106 microbiology Antibiotics Microbial Sensitivity Tests Microbiology 03 medical and health sciences chemistry.chemical_compound Daptomycin Vancomycin medicine Humans Pharmacology (medical) Pharmacology Clindamycin Drug Synergism biochemical phenomena metabolism and nutrition Antimicrobial Anti-Bacterial Agents Ciprofloxacin Infectious Diseases chemistry Biofilms Linezolid Gentamicin Gentamicins Drug Antagonism medicine.drug |
| Zdroj: | Dall, G F, Tsang, J, Gwynne, P, MacKenzie, S, Simpson, H, Breusch, S & Gallagher, M 2018, ' Unexpected synergistic and antagonistic antibiotic activity against Staphylococcus biofilms ', Journal of Antimicrobial Chemotherapy, vol. 73, no. 7, pp. 1830–1840 . https://doi.org/10.1093/jac/dky087 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | ObjectivesTo evaluate putative anti-staphylococcal biofilm antibiotic combinations used in the management of periprosthetic joint infections (PJIs).MethodsUsing the dissolvable bead biofilm assay, the minimum biofilm eradication concentration (MBEC) was determined for the most commonly used antimicrobial agents and combination regimens against staphylococcal PJIs. The established fractional inhibitory concentration (FIC) index was modified to create the fractional biofilm eradication concentration (FBEC) index to evaluate synergism or antagonism between antibiotics.ResultsOnly gentamicin (MBEC 64 mg/L) and daptomycin (MBEC 64 mg/L) were observed to be effective antistaphylococcal agents at clinically achievable concentrations. Supplementation of gentamicin with daptomycin, vancomycin or ciprofloxacin resulted in a similar or lower MBEC than gentamicin alone (FBEC index 0.25–2). Conversely, when rifampicin, clindamycin or linezolid was added to gentamicin, there was an increase in the MBEC of gentamicin relative to its use as a monotherapy (FBEC index 8–32).ConclusionsThis study found that gentamicin and daptomycin were the only effective single-agent antibiotics against established Staphylococcus biofilms. Interestingly the addition of a bacteriostatic antibiotic was found to antagonize the ability of gentamicin to eradicate Staphylococcus biofilms. |
| Databáze: | OpenAIRE |
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