Th17 cell frequency and IL-17A production in peripheral blood of patients with non-small-cell lung cancer

Autor: Jun-Ning Sun, Shiping Guo, Jing-Jing Duan, Gang Chen, Wen Su, Junsheng Li, Yan-Feng Wang, Xiao-Tang Yang, Peigang Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Medicine (General)
Lung Neoplasms
medicine.medical_treatment
Cell
Clinical marker
Enzyme-Linked Immunosorbent Assay
Biochemistry
T-Lymphocytes
Regulatory
Flow cytometry
03 medical and health sciences
0302 clinical medicine
R5-920
Carcinoma
Non-Small-Cell Lung
medicine
Humans
Lung cancer
clinical marker
030102 biochemistry & molecular biology
medicine.diagnostic_test
business.industry
flow cytometry
Biochemistry (medical)
Interleukin-17
Interleukin
Cell Biology
General Medicine
medicine.disease
T-helper17 lymphocyte
Peripheral blood
enzyme linked immunosorbent assay
medicine.anatomical_structure
Cytokine
030220 oncology & carcinogenesis
interleukin-17 cytokine
Cancer research
Cytokines
Th17 Cells
Non small cell
business
Non-small-cell lung cancer
Retrospective Clinical Research Report
Zdroj: The Journal of International Medical Research
Journal of International Medical Research, Vol 48 (2020)
ISSN: 1473-2300
0300-0605
Popis: Objective This study investigated the frequency of T-helper (Th)17 lymphocytes and production of cytokine interleukin (IL)-17 in peripheral blood of patients with non-small-cell lung cancer (NSCLC) and their use as a marker of clinical value. Methods Sixty patients with NSCLC and 60 healthy volunteers were enrolled in the study. Flow cytometry was used to detect the frequency of Th17 lymphocytes in peripheral blood, and enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of IL-17. We analyzed the association of Th17 lymphocytes and IL-17 levels in the peripheral blood of patients with their clinicopathological features. Results Frequency of Th17 lymphocytes and production of IL-17 were significantly higher in the NSCLC group than in the control group and were higher in patients with a smoking history compared with non-smokers. Moreover, Th17 lymphocyte and IL-17 expression levels were higher in patients with squamous cell carcinoma than in patients with adenocarcinoma, and significantly higher in patients with stage III and IV cancers than in patients at stage I or II. Conclusion Th17 lymphocytes and IL-17 play an important role in the development of NSCLC in patients and may have clinical value as markers for treatment of NSCLC.
Databáze: OpenAIRE
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