The impact of dehydration and hyperthermia on circulatory glutathione metabolism after exercise in the heat with insights into the role of erythrocytes
| Autor: | Orlando Laitano, Alvaro Reischak-Oliveira, Gabriela Cristina dos Santos, Denise de Melo-Marins, Alexandra Ferreira Vieira, Samuel Vargas Munhoz, Juliano Boufleur Farinha, Franccesco Pinto Boeno, Mauricio Krause |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Hyperthermia
medicine.medical_specialty Temperatura alta Antioxidant medicine.medical_treatment Science Hydration Exercício físico medicine.disease_cause Redox Eritrócitos Article General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Internal medicine medicine oxidative stress Dehydration Hipertermia Exercise Ecology Evolution Behavior and Systematics Sistema cardiovascular exercise Paleontology Glutathione medicine.disease Heat In vitro Endocrinology chemistry Desidratação Space and Planetary Science Oxidative stress Circulatory system Metabolismo [Glutationa] heat hydration |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Life, Vol 11, Iss 1144, p 1144 (2021) Life Volume 11 Issue 11 |
| Popis: | Background: Reduced glutathione (GSH) is one of the main thiols involved in antioxidant defense. Changes in circulatory levels of GSH during exercise are associated with hyperthermia and dehydration. The mechanisms by which these alterations occur are not entirely known. We hypothesize that erythrocytes could be an important source of circulatory GSH during heat stress conditions. We performed two separate experiments to address this hypothesis. Methods: In the first experiment, we sought to investigate the impact of exercise in the heat and dehydration on erythrocyte levels of GSH. A total of 10 men performed 60 min of cycling at 60% VO2peak in the heat (38.0 ± 0.9 °C) or in a control temperate environment (23.0 ± 1.0 °C), both with and without dehydration. Relative humidity ranged from 50 to 70%. Blood samples were taken before and after exercise to measure GSH and oxidized (GSSG) glutathione. In the second experiment, erythrocytes were isolated from blood samples taken at rest and heated in vitro to determine the impact of heat on erythrocyte glutathione content. Tubes with erythrocytes were exposed to water baths at different temperatures one tube was exposed to a water bath at 35 °C and the other tube to a water bath at 41 °C for a period of 30 min. After exposure to heat, plasma and erythrocytes were extracted for GSH and GSSG analyses. Results: Dehydration decreased circulatory GSH, regardless of ambient temperature (temperate and heat decreased 15.35% and 30.31%, respectively), resulting in an altered redox balance. Heat increased GSH levels in vitro. Conclusion: Our data suggest that dehydration decreases circulatory GSH levels regardless of environmental temperature. In addition, in vitro data suggests that erythrocytes may contribute to the release of GSH during exposure to heat stress. |
| Databáze: | OpenAIRE |
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