Exposure to chlorpyrifos at different ages triggers APOE genotype-specific responses in social behavior, body weight and hypothalamic gene expression
| Autor: | Fernando Sánchez-Santed, Maria Cabré, Fiona Peris-Sampedro, Pia Basaure, José L. Domingo, Jordi Blanco, Maria Teresa Colomina, Judit Biosca-Brull, Laia Guardia-Escote |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Apolipoprotein E
Male medicine.medical_specialty Insecticides Genotype Apolipoprotein E4 Hypothalamus Gene Expression Mice Transgenic 010501 environmental sciences Biology Body weight 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Apolipoproteins E Orexigenic Internal medicine Gene expression medicine Animals 030212 general & internal medicine Receptor Social Behavior 0105 earth and related environmental sciences General Environmental Science Behavior Animal Body Weight Neuropeptide Y receptor Mice Inbred C57BL Endocrinology chemistry Chlorpyrifos medicine.drug |
| Zdroj: | Environmental research. 178 |
| ISSN: | 1096-0953 |
| Popis: | To date, we have shown that apolipoprotein E (APOE) polymorphisms differentially modulate the neurobehavioral and metabolic effects of chlorpyrifos (CPF), a widely used pesticide, which is detected as residue in food. We previously reported that, after being exposed to CPF, APOE3 subjects exhibit metabolic dysfunctions while APOE4 subjects undergo changes in behavior. In the current study, we investigated the effects of a double exposure to CPF on social behavior and hypothalamic gene expression in apoE-targeted replacement (TR) mice. Male apoE3-and apoE4-TR mice were exposed to CPF at 0 or 1 mg/kg/day on postnatal days 10–15 and then, during adulthood (5 months of age), fed a CPF-supplemented diet (0 or 2 mg/kg/day) for 15 days. During adult exposure to CPF, body weight gain and food intake were monitored. At the end of the adult exposure period, we evaluated social behavior in a three-chamber test, as well as mRNA levels of hypothalamic neuropeptides and receptors related to social behavior and feeding control. Adult CPF exposure increased food intake in general, but only apoE4 mice increased their body weight. Postnatal CPF exposure improved preference for the social contexts in apoE4 mice while adult CPF exposure did the same in apoE3 mice. Anorexigenic-peptide and social-related behavior gene expression decreased as a result of adult CPF exposure in apoE4 mice, and neuropeptide Y was more expressed in apoE4 mice. These results indicate that CPF exposure produces orexigenic and metabolic effects and enlarges individual differences in social behavior, especially in apoE3 mice. |
| Databáze: | OpenAIRE |
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