Point-of-Care Adipose-Derived Stromal Vascular Fraction Cell Isolation and Expanded Polytetrafluoroethylene Graft Sodding
| Autor: | Leigh B. Kleinert, Stuart K. Williams, Kevin D. Lye, Charles B. Ross, Gary D Gentzkow, Paul E. Kosnik, Amit J Dwevidi, Marvin E. Morris |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Pathology medicine.medical_specialty Tissue-Engineered Vascular Graft Point-of-Care Systems Cell Biomedical Engineering Medicine (miscellaneous) Adipose tissue Transplants Bioengineering Expanded polytetrafluoroethylene Cell Separation 030204 cardiovascular system & hematology 03 medical and health sciences Automation 0302 clinical medicine Medicine Humans Cell isolation Polytetrafluoroethylene Point of care Aged Ultrasonography business.industry Stromal vascular fraction Middle Aged medicine.anatomical_structure Treatment Outcome Adipose Tissue 030220 oncology & carcinogenesis Female Stromal Cells business |
| Zdroj: | Tissue engineering. Part C, Methods. 23(8) |
| ISSN: | 1937-3392 |
| Popis: | Adipose-derived stromal vascular fraction (SVF) cell populations are being evaluated for numerous clinical applications. The current study evaluated a point-of-care technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived SVF cells to be used in the fabrication of a tissue-engineered vascular graft in the operating room. A total of seven patients were enrolled in this study and received femoral to tibial expanded polytetrafluoroethylene bypass grafts to treat peripheral arterial disease. Lipoaspiration of fat was performed on five patients, and the fat sample was processed immediately in the automated system in the operating room. The mean processing time, from the point of fat delivery into the instrument to removal of the SVF-containing syringe, was 70 min. The SVF cell population was evaluated for cell yield, cell viability, endotoxin levels, and microbial contamination. Samples of the SVF preparation were further subjected to microbiologic evaluation both microscopically before implantation of the graft and through a microbiologic screening using aerobic and anaerobic culture conditions. Mean cell yield was 1E5 cells per cc of fat, and endotoxin levels were below the FDA recognized standards. All SVF preparations were released for graft preparation, and the intimal surface of 90-cm-long grafts was pressure sodded with cells at a concentration of 2E5 cells/cm |
| Databáze: | OpenAIRE |
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