Rio Tinto as a niche for acidophilus enzymes of industrial relevance

Autor: Abdelali Daddaoua, Consolación Álvarez, Monika Oggerin, Nuria Rodriguez, Estrella Duque, Ricardo Amils, Jean Armengaud, Ana Segura, Juan Luis Ramos
Rok vydání: 2023
Předmět:
Zdroj: Microbial Biotechnology. 16:1069-1086
ISSN: 1751-7915
Popis: Lignocellulosic residues are amongst the most abundant waste products on Earth. Therefore, there is an increasing interest in the utilization of these residues for bioethanol production and for biorefineries to produce compounds of industrial interest. Enzymes that breakdown cellulose and hemicellulose into oligomers and monosaccharides are required in these processes and cellulolytic enzymes with optimum activity at a low pH area are desirable for industrial processes. Here, we explore the fungal biodiversity of Rıo Tinto, the largest acidic ecosystem on Earth, as far as the secretion of cellulolytic enzymes is concerned. Using colorimetric and industrial substrates, we show that a high proportion of the fungi present in this extremophilic environment secrete a wide range of enzymes that are able to hydrolyze cellulose and hemicellulose at acidic pH (4.5–5). Shotgun proteomic analysis of the secretomes of some of these fungi has identified different cellulases and hemicellulolytic enzymes as well as a number of auxiliary enzymes. Supplementation of pre-industrial cocktails from Myceliophtora with Rio Tinto secretomes increased the amount of monosaccharides released from corn stover or sugar cane straw. We conclude that the Rio Tinto fungi display a good variety of hydrolytic enzymes with high industrial potential.
Consejo Superior de Investigaciones Cientificas PTI- SusPlast+ ref 2021 AEP084
European Commission
Ministry of Science and Innovation, Spain (MICINN)
Instituto de Salud Carlos III
Spanish Government RTI2018-094370- B- I00
Databáze: OpenAIRE
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