Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films
| Autor: | Rylan Kautz, Justin P. Kerr, Atrouli Chatterjee, Janahan Arulmoli, Parsa Derakhshan, Long Phan, Lisa A. Flanagan, Alex Allevato, Jessica E. Leal-Cruz, Alon A. Gorodetsky, Mahan Naeim, LeAnn Le, James Long, Francesco Tombola |
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| Rok vydání: | 2021 |
| Předmět: |
Letter
0206 medical engineering Biomedical Engineering 02 engineering and technology Reflectin cephalopods heparin Mice Neural Stem Cells Animals Progenitor cell Cell adhesion Cell Proliferation patterning biology Cell growth Chemistry Structural protein Proteins Cell Differentiation cell adhesion Adhesion 021001 nanoscience & nanotechnology 020601 biomedical engineering Neural stem cell Cell biology Cephalopoda biology.protein Stem cell 0210 nano-technology biomaterials |
| Zdroj: | ACS Biomaterials Science & Engineering ACS biomaterials science & engineering, vol 6, iss 3 |
| ISSN: | 2373-9878 |
| Popis: | Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material. |
| Databáze: | OpenAIRE |
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