A DNA-RNA-protein complex isolated from rat liver
| Autor: | Leonard J. Cole, Karl F. Swingle |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
In Vitro Techniques
Histones chemistry.chemical_compound Ribonucleases Structural Biology medicine Animals Deoxyribonuclease II Ribonuclease Molecular Biology Deoxyribonucleases biology RNA DNA Trypsin Molecular biology Rats Liver chemistry Biochemistry Spectrophotometry Sephadex Chromatography Gel biology.protein Ultracentrifuge Deoxyribonuclease I Ultracentrifugation medicine.drug |
| Zdroj: | Journal of Molecular Biology. 15:573-586 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/s0022-2836(66)80128-6 |
| Popis: | Deoxyribonucleic acid isolated from rat liver by the p -aminosalicylate-phenol method, but without ribonuclease treatment, contained 30 to 62% of ribonucleic acid and 1 to 10% protein. These were attached to the DNA in a manner which affected its centrifugal behavior, its gel filtration pattern and its reaction with deoxyribonuclease II but not with deoxyribonuclease I. DNA isolated similarly from rat spleen, thymus, or kidney had only traces of RNA associated with it. Artificial mixtures of DNA, RNA and histone did not behave like the complex from liver with respect to the action of DNase II, nor in the centrifuge, nor on filtration through a Sephadex gel column. The thermal denaturation curve of the artificial mixture was similar to that of a sample of liver complex of analogous composition. The RNA of the complex was readily attacked by ribonuclease, and the complex was dissociated by the action of trypsin. |
| Databáze: | OpenAIRE |
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