SPaCE Swab: Point-of-Care Sensor for Simple and Rapid Detection of Acute Wound Infection
| Autor: | June D. Mercer-Chalmers, Anthony Sack, Simon Booth, Amber Young, A.T.A. Jenkins, Rosemary Greenwood, Karen Coy, Naing Tun Thet |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
liposomes
Virulence Bioengineering 02 engineering and technology medicine.disease_cause 01 natural sciences Enterococcus faecalis Article biofilm Microbiology Sepsis Medicine Candida albicans Instrumentation Fluid Flow and Transfer Processes biology integumentary system business.industry Pseudomonas aeruginosa Process Chemistry and Technology 010401 analytical chemistry Biofilm bacterial infection infection detection 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease 0104 chemical sciences wounds Staphylococcus aureus point-of-care fluorescent dye 0210 nano-technology business Bacteria |
| Zdroj: | ACS Sensors |
| ISSN: | 2379-3694 |
| Popis: | Wound infection is commonly observed after surgery and trauma but is difficult to diagnose and poorly defined in terms of objective clinical parameters. The assumption that bacteria in a wound correlate with infection is false; all wounds contain microorganisms, but not all wounds are clinically infected. This makes it difficult for clinicians to determine true wound infection, especially in wounds with pathogenic biofilms. If an infection is not properly treated, pathogenic virulence factors, such as rhamnolipids from Pseudomonas aeruginosa, can modulate the host immune response and cause tissue breakdown. Life-threatening sepsis can result if the organisms penetrate deep into host tissue. This communication describes the sensor development for five important clinical microbial pathogens commonly found in wounds: Staphylococcus aureus, P. aeruginosa, Candida albicans/auris, and Enterococcus faecalis (the SPaCE pathogens). The sensor contains liposomes encapsulating a self-quenched fluorescent dye. Toxins, expressed by SPaCE infecting pathogens in early-stage infected wounds, break down the liposomes, triggering dye release, thus changing the sensor color from yellow to green, an indication of infection. Five clinical species of bacteria and fungi, up to 20 strains each (totaling 83), were grown as early-stage biofilms in ex vivo porcine burn wounds. The biofilms were then swabbed, and the swab placed in the liposome suspension. The population density of selected pathogens in a porcine wound biofilm was quantified and correlated with colorimetric response. Over 88% of swabs switched the sensor on (107-108 CFU/swab). A pilot clinical study demonstrated a good correlation between sensor switch-on and early-stage wound infection. |
| Databáze: | OpenAIRE |
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