Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe
| Autor: | Boeun Lee, Jeehyeon Bae, Byunghee Hwang, Tae-Il Kim, Youngmi Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Eosinophil Peroxidase Hypochlorous acid Surface Properties 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Catalysis Mice 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry Immune system Hypobromous acid medicine Animals Particle Size Fluorescent Dyes Molecular Structure biology Bromates Optical Imaging General Chemistry Dipstick Hypochlorous Acid 0104 chemical sciences Kinetics RAW 264.7 Cells 030104 developmental biology chemistry Cancer cell biology.protein Eosinophil peroxidase Oxidative stress |
| Zdroj: | Journal of the American Chemical Society. 140:11771-11776 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.8b07073 |
| Popis: | The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported. |
| Databáze: | OpenAIRE |
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