Baculovirus-expressed muscovy duck reovirus sigmaC protein induces serum neutralizing antibodies and protection against challenge
| Autor: | Martine Cherbonnel, André Jestin, Véronique Jestin, Gaëlle Kuntz-Simon, Philippe Blanchard |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Virus Cultivation viruses Orthoreovirus Avian Genetic Vectors Reoviridae Biology Antibodies Viral Virus Antibody Specificity Neutralization Tests Animals Pericarditis Orthoreovirus Poultry Diseases Vaccines Synthetic General Veterinary General Immunology and Microbiology Immunogenicity Vaccination Public Health Environmental and Occupational Health RNA-Binding Proteins Viral Vaccines Tenosynovitis Fibroblasts biology.organism_classification Virology Nucleopolyhedroviruses Reoviridae Infections Specific Pathogen-Free Organisms Infectious Diseases Ducks Immunization Humoral immunity biology.protein Molecular Medicine Capsid Proteins Female Antibody |
| Zdroj: | Vaccine. 20(25-26) |
| ISSN: | 0264-410X |
| Popis: | Recombinant baculoviruses with sigmaC- or sigmaB-encoding gene from muscovy duck reovirus (DRV) were constructed. Western-blot analysis showed that sigmaC was more immunoreactive than sigmaB. Vaccination of SPF ducks with two injections, 3 weeks apart, of emulsions containing sigmaC or sigmaC + sigmaB elicited DRV-specific neutralizing antibodies. Following challenge, vaccination partially--or even totally in some cases--prevented the appearance of clinical symptoms. Moreover, immunization reduced the severity of reovirus-induced tenosynovitis and prevented pericarditis development during the course of the assay. Thus, DRV sigmaC, alone or co-expressed with sigmaB, appeared as a good candidate for vaccination of ducks (96/100 mots). |
| Databáze: | OpenAIRE |
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