Polymodal Transient Receptor Potential Vanilloid (TRPV) Ion Channels in Chondrogenic Cells
| Autor: | Csaba Matta, Ádám Roland Takács, Csilla Somogyi, Éva Katona, Pál Gergely, Zsofia Foldvari, Tibor Hajdú, Tamás Juhász, Róza Zákány, Nóra Dobrosi |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Hot Temperature
Cell Culture Techniques Chick Embryo TRPV Weight-Bearing lcsh:Chemistry Mice Transient receptor potential channel 0302 clinical medicine Elméleti orvostudományok lcsh:QH301-705.5 cartilage formation Cells Cultured Spectroscopy 0303 health sciences Chemistry high density culture General Medicine Orvostudományok Computer Science Applications Cell biology medicine.anatomical_structure Biochemistry chondrocyte Chondrogenesis heat stimulation TRPV4 TRPV6 TRPV2 TRPV1 TRPV Cation Channels Article Catalysis Chondrocyte Inorganic Chemistry 03 medical and health sciences Chondrocytes medicine Animals RNA Messenger Physical and Theoretical Chemistry Molecular Biology micromass 030304 developmental biology mechanical loading Organic Chemistry Cartilage lcsh:Biology (General) lcsh:QD1-999 Transcriptome 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences Volume 16 Issue 8 Pages 18412-18438 International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 18412-18438 (2015) |
| Popis: | Mature and developing chondrocytes exist in a microenvironment where mechanical load, changes of temperature, osmolarity and acidic pH may influence cellular metabolism. Polymodal Transient Receptor Potential Vanilloid (TRPV) receptors are environmental sensors mediating responses through activation of linked intracellular signalling pathways. In chondrogenic high density cultures established from limb buds of chicken and mouse embryos, we identified TRPV1, TRPV2, TRPV3, TRPV4 and TRPV6 mRNA expression with RT-PCR. In both cultures, a switch in the expression pattern of TRPVs was observed during cartilage formation. The inhibition of TRPVs with the non-selective calcium channel blocker ruthenium red diminished chondrogenesis and caused significant inhibition of proliferation. Incubating cell cultures at 41 °C elevated the expression of TRPV1, and increased cartilage matrix production. When chondrogenic cells were exposed to mechanical load at the time of their differentiation into matrix producing chondrocytes, we detected increased mRNA levels of TRPV3. Our results demonstrate that developing chondrocytes express a full palette of TRPV channels and the switch in the expression pattern suggests differentiation stage-dependent roles of TRPVs during cartilage formation. As TRPV1 and TRPV3 expression was altered by thermal and mechanical stimuli, respectively, these are candidate channels that contribute to the transduction of environmental stimuli in chondrogenic cells. |
| Databáze: | OpenAIRE |
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