First isolation of bacteriophages for a spirochaete: Potential genetic tools for Leptospira
| Autor: | G. Baranton, I. Saint Girons, Danielle Margarita, P. Amouriaux |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Electrophoresis
Agar Gel Leptospira Virulence biology Nucleic Acid Hybridization DNA General Medicine biology.organism_classification Microbiology Molecular Weight Bacteriophage Microscopy Electron Restriction enzyme Lytic cycle Lysogenic cycle Leptospiraceae Spirochaete Bacteriophages Molecular Biology Leptospira interrogans |
| Zdroj: | Research in Microbiology. 141:1131-1138 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/0923-2508(90)90086-6 |
| Popis: | Three bacteriophages of the saprophytic aquicole bacterium Leptospira biflexa were isolated from sewage waters from the outskirts of Paris, France. These phages do not infect representative strains of the pathogenic species Leptospira interrogans, and their host range is restricted to serovar patoc of the saprophytic species. The phages were found to be lytic and no lysogenic state could be demonstrated. Electron micrographs showed that the phages were morphologically identical and had polyhedral heads and contractile tails. Their genomes were sensitive to restriction enzymes and consisted of double-stranded DNA. Pulsed-field agarose gel electrophoresis indicated that their genomes were linear: 60 kb for LE1 and 50 kb for LE3 and LE4. |
| Databáze: | OpenAIRE |
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