| Autor: |
Broce, Sean, Hensley, Lisa, Tomoharu Sato, Lehrer-Graiwer, Joshua, Essrich, Christian, Edwards, Katie, Pajda, Jacqueline, Davis, Christopher, Bhadresh, Rami, Hurt, Clarence, Freeman, Beverly, Vishwanath Lingappa, Kelleher, Colm, Karpuj, Marcela |
| Rok vydání: |
2016 |
| DOI: |
10.6084/m9.figshare.c.3611000_d6 |
| Popis: |
Conversion of the IOAS into IHOS by nonradioactive CFPS-products is not affected by Apyrase. Glycerol-gradient fractionation profiles of the radioactive material generated in CFPs under conditions favoring intermediate-assembly structures and “chased” by the addition of nonradioactive products for 120 min and carried out in the presence or absence of the ATP inhibitor Apyrase (final concentration of 0.5 U/μl in the cell-free reaction mixture) at 37 °C. Note that in all cases, in addition to the chase at 26 °°C, the material in peak I (intermediate assembly structures) was converted to the highly ordered assembly structures (Peak II), indicating that the process of RVFV NP assembly is indeed independent of energy but is temperature dependent. (PDF 88 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
