Decreased basal chloride secretion and altered cystic fibrosis transmembrane conductance regulatory protein, Villin, GLUT5 protein expression in jejunum from leptin-deficient mice
| Autor: | Lana Leung, Jonathan Kang, Esa Rayyan, Layla Al-Nakkash, Scott Cochran, Tom L. Broderick, Justin Willey, Ryan Jelinek, Brennan Barrett, Ashesh Bhakta, David Larsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Ouabain Jejunum Basal (phylogenetics) ob/ob Internal medicine Internal Medicine medicine Targets and Therapy [Diabetes Metabolic Syndrome and Obesity] Original Research 2. Zero hunger Pharmacology biology obese diabetes Leptin intestinal secretion Small intestine 3. Good health medicine.anatomical_structure Endocrinology transport biology.protein GLUT2 Villin GLUT5 small intestine medicine.drug |
| Zdroj: | Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy |
| ISSN: | 1178-7007 |
| Popis: | Lana Leung, Jonathan Kang, Esa Rayyan, Ashesh Bhakta, Brennan Barrett, David Larsen, Ryan Jelinek, Justin Willey, Scott Cochran, Tom L Broderick, Layla Al-NakkashDepartment of Physiology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USAAbstract: Patients with diabetes and obesity are at increased risk of developing disturbances in intestinal function. In this study, we characterized jejunal function in the clinically relevant leptin-deficient ob/ob mouse, a model of diabetes and obesity. We measured transepithelial short circuit current (Isc), across freshly isolated segments of jejunum from 12-week-old ob/ob and lean C57BL/6J (female and male) mice. The basal Isc was significantly decreased (~30%) in the ob/ob mice (66.5±5.7 µA/cm2 [n=20]) (P< 0.05) compared with their lean counterparts (95.1±9.1 µA/cm2 [n=19]). Inhibition with clotrimazole (100 µM, applied bilaterally) was significantly reduced in the ob/ob mice (−7.92%±3.67% [n=15]) (P |
| Databáze: | OpenAIRE |
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