Metabolomics driven analysis of Erythrina lysistemon cell suspension culture in response to methyl jasmonate elicitation
| Autor: | Sawsan El-Masry, Hattem Mekky, Mohamed A. Farag |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Metabolite Flavonoid Ultra-performance liquid chromatography Secondary metabolite Methyl jasmonate 01 natural sciences Flavones chemistry.chemical_compound medicine Metabolomics General lcsh:Science (General) Oleanolic acid Erythrina ComputingMethodologies_COMPUTERGRAPHICS chemistry.chemical_classification lcsh:R5-920 Multidisciplinary Chromatography biology 010405 organic chemistry Erythrina lysistemon biology.organism_classification 0104 chemical sciences chemistry Biochemistry Original Article Cell culture lcsh:Medicine (General) 010606 plant biology & botany medicine.drug lcsh:Q1-390 |
| Zdroj: | Journal of Advanced Research, Vol 7, Iss 5, Pp 681-689 (2016) Journal of Advanced Research |
| ISSN: | 2090-1224 2090-1232 |
| Popis: | Graphical abstract An MS-based metabolomic approach was used to profile the secondary metabolite of the ornamental plant Erythrina lysistemon via ultra-performance liquid chromatography coupled to photodiode array detection and high resolution q-TOF mass spectrometry (UPLC-PDA-MS). Cultures maintained the capacity to produce E. lysistemon flavonoid subclasses with pterocarpans amounting for the most abundant ones suggesting that it could provide a resource of such flavonoid subclass. In contrast, alkaloids, major constituents of Erythrina genus, were detected at trace levels in suspension cultures. Methyl jasmonate (MeJA), phytohormone, was further supplied to culture with the aim of increasing secondary metabolites production and with metabolite profiles subjected to multivariate data analysis to evaluate its effect. Results revealed that triterpene i.e. oleanolic acid and fatty acid i.e. hydroxy-octadecadienoic acid were elicited in response to methyl jasmonate, whereas pterocarpans i.e., isoneorautenol showed a decline in response to elicitation suggesting for the induction of terpenoid biosynthetic pathway and concurrent with a down regulation of pterocarpans. In conclusion, a total of 53 secondary metabolites including 3 flavones, 12 isoflavones, 4 isoflavanones, 4 alkaloids, 11 pterocarpans, and 5 phenolic acids were identified. |
| Databáze: | OpenAIRE |
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