Sevoflurane inhibits neuroblastoma cell proliferation and invasion and induces apoptosis by miR‐144‐3p/YAP1 axis

Autor:	Ke He, Jun Shen, Longqiu Yang, Shudong Yao, Yiqiang Zhang
Rok vydání:	2021
Předmět:	viruses Apoptosis Toxicology Flow cytometry Neuroblastoma Sevoflurane Cell Movement Cell Line Tumor medicine Humans Cell Proliferation Pharmacology Reporter gene medicine.diagnostic_test Cell growth Chemistry YAP-Signaling Proteins General Medicine Transfection respiratory system medicine.disease Molecular biology MicroRNAs Cell culture Cancer cell Apoptosis Regulatory Proteins
Zdroj:	Basic & Clinical Pharmacology & Toxicology. 129:297-307
ISSN:	1742-7843 1742-7835
DOI:	10.1111/bcpt.13629
Popis:	BACKGROUND Sevoflurane (SEV) is a typical volatile anaesthetic and has an antitumour activity in various cancer cells. Here, we were curious whether SEV has tumour-suppressive effects in neuroblastoma (NB). METHODS NB cell lines (K-N-SH and SK-N-AS) were treated with SEV (1%, 2% and 4%). Cell Counting Kit-8 (CCK8) and Transwell assays were conducted to examine cell proliferation and invasion, respectively. The apoptosis was verified by flow cytometry, and the yes-associated protein 1 (YAP1), Bax, Bcl2 and cleaved caspase3 levels were detected by western blotting. Quantitative real-time PCR (qRT-PCR) was conducted to monitor the miR-144-3p level in SEV-treated NB cells. The targeted relationship between miR-144-3p and YAP1 was predicted by bioinformatics and testified by the dual-luciferase reporter assay. RESULTS SEV mitigated NB cell proliferation and invasion and strengthened apoptosis dose-dependently. SEV upregulated miR-144-3p. Moreover, the miR-144-3p inhibitor transfection significantly reduced the tumour-suppressive effect of SEV on NB cells. Furthermore, the dual-luciferase reporter assay confirmed that miR-144-3p targeted YAP1 and overexpressing YAP1 partially weakened the inhibitive effects of miR-144-3p on NB cells. CONCLUSION SEV abated NB cell proliferation and invasion and accelerated apoptosis through the miR-144-3p/YAP1 axis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87c2fffb890c3cda18bd754a85864c58 https://doi.org/10.1111/bcpt.13629 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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